于雪梅 朱振宇 傅祖植 钟光恕 程桦 马涧泉 510120 广州中山医科大学孙逸仙纪念医院内分泌科 中华内分泌代谢杂志 1999 0 15 3


    关键词：肥胖；瘦素；分子克隆 期刊 zhnfmdxzz 0 论著 fur -->


    

【摘要 】 目的 为了获得大量的、可供实验研究和临床应用的人leptin。方法 采用基因工程技术，把肥胖基因(obesegene, OB)编码人leptin的cDNA序列(OB1)定向插入高效原核细胞表达载体pBV221，构建重组质粒pBV221OB1，并转化大肠杆菌TG1。结果 经过限制性内切酶分析和PCR技术鉴定，重组质粒pBV221OB1含有编码人leptin的基因序列。结论 成功的构建了人leptin基因原核细胞表达体系。

Cloning human leptin gene into anE. Coli expression vector

YU Xuemei,ZHU Zhenyu,FU Zuzhi,et al.

    Department of Endocrinology,Sun Yat-sen Memorial Hospital,Sun Yat-sen University ofMedical Sciences,Guangzhou,510120

【Abstract 】 Objective Toget human leptin which can be used in experimental study and clinical application. Methods Withgene engineering technique, human leptin cDNA sequence (OB1) was inserted directly intopBV221, a proeukaryotic cell expression vector, to construct recombinant pBV221OB1 and totransform E. coli strain TG1. Results Through the analysis ofrestrictive endonuclease and polymerase chain reaction (PCR), recombinant pBV221OB1contained the cDNA sequence coding human leptin. Conclusion Proeukaryoticcell expression system containing the cDNA sequence of human leptin gene was constructed.

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