陈明 黄颂敏 曾瑜 刘聪 田青 陈明 曾瑜 646000 四川泸州医学院附院肾内科；黄颂敏 刘聪 田青 四川华西医科大学附一院肾内科 中华内分泌代谢杂志 1999 0 15 3


    关键词：糖尿病肾病；内皮素；基因 期刊 zhnfmdxzz 0 论著 fur -->


    

【摘要 】 目的 探讨内皮素-1及其mRNA在早期实验性糖尿病大鼠肾脏中的含量和意义。方法 采用免疫组织化学、原位杂交技术检测糖尿病大鼠肾组织中ET-1及其mRNA。结果 ET-1及ET-1mRNA分布一致，主要分布于肾小球内皮细胞、系膜细胞、血管平滑肌细胞及肾小管上皮细胞之中，髓质高于皮质，二者在糖尿病发生后1～2周肾小球内均明显减少(P<0.05)，4周时开始增加(P<0.05)，而在髓质中则持续增加。结论 ET-1参与了早期实验性糖尿病大鼠肾小球高灌注及肾小管间质损伤。

Content of ET-1 and its mRNA in thekidney of diabetic rats at early stages

CHEN Ming,HUAN Songmin,ZENG Yu,et al.

    Department of Nephrology,The Affiliated Hosptial,Luzhou Medical College, Si Chuan, 6466000

【Abstract 】 Objective Toobserve the content of ET-1 and its mRNA in kidney of diabetic rats at early stages andits significance. Methods Diabetes mellitus was induced by aninterperitoneal injection of streptozotocin (55mg/kg). The content of ET-1 was examined byimmunohistochemical stainning and then half-quantitative analysis by Mia-2000 pathologycomputer image analyser while the content of ET-1 mRNA was observed by in situhybridizational technique. Results The distribution of ET-1 and itsmRNA were similar. The postive signal of ET-1 and ET-1 mRNA were observed in glomerularendothelial, mesangial, vascular smooth muscle and renal tubular epithelial cells. Inglomeruli of the 1st-2nd weeks, ET-1 and ET-1 mRNA were decreased significantly comparedwith the normal control (P<0.05). But in tubulointerstitial segment of diabetic rats,they were increased as compared with the normal control. Conclusion ET-1may play a role in the pathogenesis of glomerular hyperinfusion and tubulointerstitialinjury.

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