汤洋 畅继武 隋志芳 马腾骧 300211 天津医科大学第二医院泌尿外科(汤洋)；天津市泌尿外科研究所(畅继武、隋志芳、马腾骧) 中华泌尿外科杂志 1999 0 20 6


    关键词：细胞学技术；细胞，培养的 期刊 zhmnwkzz 0 论著 fur -->


    

【摘要】 目的 建立一种比较简便的人正常尿路上皮细胞无血清培养方法。 方法 标本组织块用冷消化法分离出尿路上皮细胞，培养于无血清生长培养基。观察传代细胞形态及生长情况，并用TGF-β₁ 抑制细胞增殖试验检验细胞在有血清或无血清培养基中对TGF-β₁ 的反应。 结果 培养的总成功率为73%，细胞具有典型的移行上皮细胞形态学特征，细胞能在24小时后增殖进入对数生长期，传代9～11次后开始衰退。另外，无血清培养体系比含血清培养体系更能反映出TGF-β₁ 对细胞抑制作用。 结论 此培养体系具有简单、短期内可扩增出大量纯净上皮细胞、应用范围广和培养成功率高的特点。

Culture of human normal urothelial cells with serum-free medium

TANG Yang,CHANG Jiwu,SUI Zhifang

et al.Department of Urology,The Second Hospital,Tianjin Urological Institute,Tianjin 300211

【Abstract】 Objective To establish a relative simple and convenient serum-free culture method of human unthelial cells. Methods Epithelial cells were isolated from sample tissue by cold trypsinization and cultured in serum-free growth medium.Growth and morphology of passaged cells were observed.Moreover,cells reacting to TGF-β ₁ was cheked with TGF-β ₁ inhibiting cell growth test in serum-free or containing serum medium. Results Total successful rate of culture was 73%.Cultured cells had typical morphological features of transitional epithelium and rapidly proliferated into logarithm growth period.After 9～ 11 passages,cells began senescence.The changes of cell proliferation in serum-free medium rather than serum containing medium reflected the TGF-β ₁ effect. Conclusions This culture system was simple and convenient,amplifying a lot of pure epithelial cells in short term with more applicability and high successful culture rate.

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