张晶 蒋宇光 朱章菱 许建军 马潞林 周艳 高居忠 刘敬忠 100020 首都医科大学附属北京红十字朝阳医院,北京基因诊断实验室遗传病分部 中华泌尿外科杂志 1999 0 20 6


    关键词：肾；移植；基因型 期刊 zhmnwkzz 0 论著 fur -->


    

【摘要】 目的 适应临床器官基因配型的需要。 方法 建立从微量全血不经抽提DNA，直接进行PCR-SSP完成HLA-DRB1位点基因分型,结果与血清学分型方法对照。部分分型结果用直接测序法(SBT)验证。 结果 50例标本DRB1配型22例与血清学分型结果一致。对于结果不一致者,采用SBT分型技术进行验证，其分型结果与用微量全血直接进行PCR-SSP的分型结果一致。 结论 从微量全血直接进行HLA-DRB1基因配型的技术准确可靠。

Trace blood HLA-DRB1 typing by means of PCR-SSP

ZHANG Jing, JIANG Yuguang, ZHU Zhangling

et al. Beijing Red Cross Chaoyang Hospital,Affiliated to Capital University of Medical Sciences, Beijing 100020

【Abstract】 Objective To establish a convenient and practical trace blood DNA typing technique. Methods Trace blood without DNA extraction was used for DNA typing by means of PCR-SSP and the results were checked by sequence-based-typing (SBT) method. Results 50 samples of trace blood were successfully typed by means of PCR-SSP and the procedure takes about 2 h.The accordance rate between the trace blood PCR-SSP and the serology method has been 68.8%. Conclusions The trace blood PCR-SSP typeing technique is reliable and accurate.

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