核酶对鸭乙型肝炎病毒感染体内抗病毒作用效果的观察
核酶|鸭乙型肝炎病毒|复制|表达,关键词：
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关键词：核酶；鸭乙型肝炎病毒；复制；表达
【摘要】 目的 观察核酶在体内抗病毒作用效果。方法 选择鸭乙型肝炎(DHBV LJ-76)及其鸭动物模型作为检测系统，将针对DHBV Pre-S 736位点的核酶(RzDS)插入pJ120质粒构建成pJ-RzDS。与痘苗病毒天坛761株(VV)同源重组后获得含RzDS的重组痘苗病毒(V-RzDS)。用DHBV感染1日龄北京鸭，分别在感染的同时、感染后24小时和72小时注射V-RzDS和VV，10天后检测血清中DHBV DNA和DHBsAg的含量。结果 北京鸭在感染DHBV的同时注射V-RzDS，其DHBV DNA和DHBsAg的含量均低于VV对照组，两组之间有显著性差异。结论 提示RzDS对DHBV基因组复制和表达均有抑制作用，其抑制率分别为45%和63%。
The effect of ribozyme RzDS on duck hepatitis B virus Li Hong,Chen Wen,Xu Wei,et al.National Laboratory of Molecular Virology and Genetic Engineering,Beijing 100052
Abstract To study the effect of ribozyme in vivo,the hammerhead ribozyme RzDS was designed and synthesized,which aims at the pregenome RNA of DHBV(LJ-76) at the 736 site of the genome.The RzDS cleaved efficiently its substrate in vitro.We constructed pJ-RzDS,in which RzDS was inserted into pJ120 plasmid under the control of Pll promoter.After homogenous recombination with the vaccinia virus,Tian Tan strain,we got the recombinant(V-RzDS) which carried RzDS.V-RzDS was injected into nestling Beijing ducks preinfected with DHBV. On the tenth day after intravenous injection,DHBV DNA and DHBsAg in serum were detected.The quantity of DHBV DNA and DHBsAg were evidently lower than that of the controls, when V-RzDS injection was simultaneously given with DHBV.It suggests that RzDS can block replication and expression of DHBV in vivo.
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