关键词：巨细胞病毒；包涵体；小鼠模型；空斑形成单位
【摘要】 目的 为了探讨巨细胞病毒的致病机理。方法 4周龄Balb/C小鼠腹腔内接种小鼠巨细胞病毒(MCMV)。结果 导致小鼠急性感染期体重下降，生长迟缓，唾液腺肿胀以至于死亡。唾液腺中检出高滴度感染性病毒(2.0×105 PFU/ml)。在小鼠3T3/Swiss albino细胞单层上形成的空斑清晰，易判断计数 、镜检组织切片可见脑神经原细胞胞浆内包涵体。结论 小鼠巨细胞病毒模型的建立为抗-CMV有效药物的筛选以及对CMV感染的预防、治疗提供了资料。
Primary studies on the establishment of cytomegalovirus murine model
Ji Yuhua,Zhu Yueqiu, Chen Shuyun, et al. Department of Clinical Laboratory,Rui Jin Hospital, Shanghai 200025
Abstuact Intraperitoneal inoculation of Murine cytomegalovirus(MCMV) into Balb/C mice at age of 4 weeks resulted in acute MCMV infection of the mice. The infected mice had body weight reduction,growth retardation,salivary gland swelling and death.A lethal dose was 5×106 PFU/ml of MCMV.Virus could be recovered from salivery gland and the infectivity titer of MCMV reached to a high titer of 2×105 PFU/ml. Plaque formation of MCMV in 3T3/Swiss albino cells wasidentified. Histopathological examination showed the appearance of MCMV-specific intracytoplasmic inclusion bodies in neuron cells of the mouse brain.
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