【摘要】 目的 获得7型腺病毒(Ad7)疫苗株87mu?97.4mu核苷酸序列，分析该区段的基因结构和功能。方法 应用Sanger双脱氧法进行核苷酸序列分析。结果 Ad7疫苗株87mu?97.4mu全长3?698个核苷酸，推测编码纤维蛋白(325个氨基酸)和E3区15.4kD蛋白，E4区5个蛋白(ORF 14.2，ORF 15.7，ORF 8.1，ORF 42.8和ORF 10.3)。结论 这一结果为阐明Ad7的基因结构与功能及利用该病毒做为基因工程疫苗载体打下一定的基础。

    Sequence analysis of Ad7 vaccine strain 87mu?97.4mu fragment Zhou Jiehao,Qu Jianguo,Shi Changxin,et al.Institute of Virology,Chinese Academy of Preventive Medicine,Bejing 100052

    Abstract Adenovirus 7 vaccine strain(Ad7v)possesses potentiality to be used as a vector for producing recombinant vaccine.In order to construct Ad7 vector,the nucleotide sequence of a 3698bp fragment(87 to 97.4 mu)of the virus DNA was analysed.The fragment encodes three ORFs namely, one ORF of early region 4(E4),one ORF of early region 3 and one ORF of fiber protein.The predicted amino acids of three ORFs(ORF 8.1kD、42.8kD and 10.3kD)share 33%,43%and 44%homology with Ad2 E4 corresponding regions (ORF 3,ORF 4 and ORF 6/7)respectively.A comparison of the nucleotide and amino acid sequences of those fiber ORF demonstrated a higher homology between Ad7v and prototype strains of Ad7v than those between Ad7v and Ad7h.The Ad7v E3 15.4kD protein shares 86%, 88%, 90% and 98.5% amino acid homology with the equivalent region of ad7h E3 15.3kD protein, Ad4 E3 region 15kD protein, Ad3 E3 15.3kD and Ad7p E3 15.3kD protein protein respectively,but only 68% with Ad2 E3 14.7kD protein.

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