万卓越 皮国华 孙宁 李志仁 唐慰萍 谷淑燕 150036 哈尔滨 黑龙江省卫生防疫站(万卓越 李志仁)；中国预防医学科学院病毒学研究所(皮国华 谷淑燕)；广东医学院病理教研室(孙宁 唐慰萍) 中华实验和临床病毒学杂志 1998 0 0 1


    关键词：鼻咽肿瘤/病毒学；基因工程抗原；酶联免疫吸附测定 期刊 zhsyhlcbdxzz 0 病毒诊断 fur -->


    

【摘要】 目的 为了建立鼻咽癌(NPC)早期诊断方法。方法 以基因工程表达的、经纯化的EB病毒(Epstein-Barr virus, EBV)早期抗原(EA)成分EA-D和EA-R作为诊断抗原，建立了酶联免疫吸附试验(ELISA)，检查30例NPC病人及49例正常人血清中的EA/IgA抗体。结果 用ELISA检测抗体较用细胞涂片免疫酶方法(IE)敏感。ELISA检测NPC病人血清中EA/lgA抗体，阳性率为100%，EA/lgA抗体效价均≥1∶100。而用IE法，平行检测30例NPC病人血清中EA/lgA抗体效价，结果6例为阴性(<1∶10)，抗体阳性率为70%。ELISA明显地提高了NPC的检出率。以p138(EA-R)和p54(EA-D)分别或混合包被，检测对EBV特异的EA-D和EA-R的抗体。结论 表明在NPC病人血清中存在对EA两种抗原的抗体，对EA-D的抗体滴度高于对EA-R的抗体。因此，以两种抗原混合包被作为诊断抗原建立的ELISA方法，为NPC的早期诊断提供更敏感、特异和简便的手段。

Early diagnosis of nasopharyngeal carcinoma using recombinant antigens expressed in bacterial

Wan Zhuoyue, Pi Guohua Sun Ning, et al. The Epidemic Prevention Station of Heilongjiang Province 150036

Abstract An enzyme-linked immunosorbent assay(ELISA) was developed by using recombinant purified early antigens, EA-D and EA-R to detect lgA antibodies in sera from nasopharyngeal carcinoma(NPC)pateints. 30 sera from NPC pateints and 49 sera from normal persons were detected by the developed ELISA. The serum positive rate and antibody titer were compared with immunoenzymatic method(IE)on slide with Raji cells Smears. ELISA is more sensitive and specific. The serum positive rate of NPC patients was increased from 70% by IE to 100% by ELISA and 77% of the serum antibody titer reached≥1∶200. The results show that the expressed recombinant p138 and p54 antigens are effective for detection of EA/IgA antibody in sera from NPC patients and ELISA will be a sensitive, specific and convenient method for early diagnosis of NPC.

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