关键词：钩端螺旋体病；传染源；染色体； 核糖体核糖核酸基因
摘 要：目的 确定耕牛是否为七 日热型、澳洲型钩端螺旋体病的传染源。方法 自钩端螺旋体病患者血液及耕牛尿中分离出的12株七日热型、澳洲型钩端螺旋体野生株染色体DNA用限 制性内切酶EcoRⅠ酶切后，进行染色体DNA限制性内切酶图谱及16S+23S rRNA基因限制性内 切酶片段长度多态性分析。结果 自钩端螺旋体病患者血液及耕牛 尿中分离的同一血清型的钩端螺旋体野生株的染色体DNA酶谱及核糖核酸型相同。血清型不 同的钩端螺旋体野生株的染色体DNA酶谱及核糖核酸型则不相同。结论 耕牛是我国七日热型及澳洲型钩端螺旋体病的传染源，这对控制我国七日热型及澳洲型 钩端螺旋体病的流行有着非常重要的现实意义。
A molecular epidemiological investigation on Leptospira inter rogans serovar hebdomadis and australia in China
SHI Manhua LIANG Zhongxing DU Xuefei, et al.
( Institute of Epidemiology and Micro biology,Chinese Academy of Preventive Medicine,Beijing 102206,China)
Abstract ：Objective In order to confirm whether cattl e serve the source of infection for patients with Leptospira interrogans serovar hebdomadis and australia. Methods 12 field strains of le ptospira interrogans serovar hebdomadis and australia were isolated from blood s amples of patients with leptospirosis and farm cattle urine. An analysis of chro mosome DNA restriction endonuclease patterns (REP) and restriction fra gments length polymorphism (RFLP) of 16S+23S rRNA gene were processed by digesti on of chromosome DNA using EcoR I. Results The same serov a r field strains of Leptospira interrogans from blood samples of patients with le ptospirosis and farm cattle urine resulted in unique restriction endonuclease pa tterns (REP) and ribosomal types (RT).The chromosome DNAs from field strains tha t belonged to different serovars of Leptospira interrogans caused different rest riction endonuclease patterns and ribosomal types. Conclusion In accordance with this results, we recognize that farm cattle serve the i nfectious source of leptospirosis serovar hebdomadis and australia thus may have realistic significance in the control of leptospirosis serovar epidemic of hebd omadis and australia in China.
Key words ： Leptospirosis; Source of infection; Chromos ome; rRNA gene
钩端螺旋体(简称钩体)病是一种流行范围十分广泛的自然疫源性疾病 ......
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