关键词：色素上皮，眼/免疫学；淋巴细胞；脱噬作用；流式细胞术
【摘要 】 目的 观察视网膜色素上皮(retinal pigment epithelium,RPE)细胞对体外抗原特异性活化淋巴细胞的影响，探索RPE细胞在眼免疫赦免中的作用。 方法 建立RPE与抗原特异性T淋巴细胞系和静止淋巴细胞之间的共培养系统。使用基因组DNA电泳、DNA末端标记和流式细胞技术检测凋亡的诱导情况。 结果 与RPE共培养的抗原特异性活化淋巴细胞出现凋亡征象。随着时间的延长，凋亡细胞的数量逐渐增加。定量分析表明：共育24小时的凋亡细胞占5.95%；48小时占9.38%；72小时占17.95%。与此相反，RPE对于静止的T细胞则不具有显著诱导凋亡的作用。 结论 RPE细胞具有诱导入侵淋巴细胞凋亡的能力。这种现象作为免疫反应的限制机制，在眼后节免疫赦免的维持和保护眼免受免疫性炎症反应中可能具有重要的意义。
中国图书资料分类法分类号 R774.1 R392.11 R446
Apoptosis of activated lymphocytes induced by retinal pigment epithelial cells in vitro
PENG Guanghua,LI Zhijie,LI Chen.
Research Section of Ophthalmology ,Institute of Tissue Transplantation ＆ Immunology,Jinan University,Guangzhou 510632,China
【Abstract 】 Objective To examine the influence of retinal pigment epithelium(RPE) cells on antigen-specific activated lymphocytes in vitro,and to explore the role of RPE cells in the immune privilege of the eye. Methods Co-culture systems of RPE cells with antigen-specific T lymphocyte lines and resting T lymphocytes were established in vitro.Induction of apoptosis was detected by genomic DNA electrophoresis,DNA in situ end-labelling and flow cytometry. Results RPE cells induced apoptosis in antigen-specific activated T lymphocytes.24 hours after culture,the signs of apoptosis appeared in lymphocytes co-incubated with RPE cells.As time of co-culture went on,the number of apoptosic cells increased.Quantitative analysis of apoptosic cells showed that apoptosic cells accounted for 5.95% after 24 hours,9.38% after 48 hours,and 17.95% after 72 hours.In contrast,RPE cells induced few apoptosis in resting T lymphocytes. Conclusions These results suggest that RPE cells possess the ability to induce the apoptosis of invading lymphocytes. This phenomenon serves as a restrain mechanism of immune response and may be of vital importance in the maintenance of immune privilege in posterior segment of eye and in the protection of eye from the damage of immunogenic inflammation.
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