侯鹏 许国铭 龚燕芳 屠振兴 李兆申 纪徐淮 200433 上海，第二军医大学长海医院消化科 中华内科杂志 1999 0 38 11


    关键词：螺杆菌，幽门；胃肠疾病；vac A基因表型 期刊 zhnkzz 0 论著 fur -->


    

【摘要】 目的 探讨用vac A基因表型检测对幽门螺杆菌(Hp)进行分型，并分析不同基因型与相关胃肠疾病之间的关系。方法 对临床胃黏膜标本中Hp菌株进行分离培养及其总RNA抽提，逆转录聚合酶链反应检测vac A基因表型。结果 50株临床分离Hp菌株，vac A基因表型s1/m2型46株(92%)，s1/m1型4株(8%)，未发现s2型。结论 上海人Hp菌株vac A基因绝大多数表达s1/m2型。s1/m2基因表型分布于所有Hp相关胃十二指肠疾病的Hp菌株中。

The genotype of vac A gene of Helicobacter pylori (Hp)

    and its correlation with the gastroduodenal diseases associated with Hp

HOU Peng， XU Guoming， GONG Yanfang， et al.

    Department of Gastroenterology, Changhai Hospital, Second

    Military Medical University , Shanghai 200433

【 Abstract 】 Objective To analyze the expression of vac A gene of Helicobacter pylori (Hp) isolated from Chinese patients and to find out the relationship between the genotype of Hp vac A and the gastroduodenal diseases associated with Hp. Methods Clones of Hp obtained from patients with gastroduodenal diseases were cultured and mRNA extraction was performed with reverse transcriptase polymerase chain reaction (RT-PCR). Results 92% of the strains isolated in Shanghai(China) was found to be s1/m2. Strains expressing s1/m1 forms were obtained in 4 cases. s2 form was not found. Conclusion The overwhelming majority of strains isolated from Shanghai patients expresses s1/m2 from. The s1/m2 genotype is dispersed in all of the gastroduodenal diseases associated with Hp.

     【 Key words 】 Helicobacter pylori Gastroentestinal diseases vac A genotype

幽门螺杆菌(Hp)与慢性胃炎、消化性溃疡、胃癌及胃恶性淋巴瘤的发生及发展有密切关系 ......