邢承忠 陈峻青 徐惠绵 110001 沈阳，中国医科大学第一临床学院肿瘤科 中华肿瘤杂志 1999 0 21 4


    关键词：胃肿瘤；顺铂；细胞凋亡；钙/代谢；肌醇1，4，5-三磷酸/代谢 期刊 zhzlzz 0 基础研究 fur -->


    

【摘要 】 目的 探讨顺铂诱导胃癌细胞凋亡过程中细胞内游离Ca²⁺ 浓度([Ca²⁺ ]i)和三磷酸肌醇(IP₃ )浓度的变化特点和意义。方法 选择顺铂诱导胃癌细胞凋亡，采用光镜、电镜、琼脂糖凝胶电泳和流式细胞仪检测凋亡。Fura-2荧光负荷技术测[Ca²⁺ ]i，竞争性蛋白结合法测定IP₃ 浓度。结果顺铂浓度为2 μg/ml作用于BGC823胃癌细胞24小时出现典型的凋亡改变。胃癌细胞凋亡过程中，胞内[Ca²⁺ ]i明显升高，以凋亡早期更为明显。胃癌细胞凋亡早期(30秒)，IP₃ 浓度明显升高，到凋亡晚期(2～24小时)，IP₃ 浓度明显降低。结论 在胃癌细胞凋亡过程中，[Ca²⁺ ]i表现为上调趋势。IP₃ 浓度在凋亡早期，表现为上调趋势，在稍晚阶段，表现为下调趋势，这为治疗胃癌提供新靶点。

Changes in [ Ca²⁺ ] i and IP₃ levels in the process of cisplatin-induced apoptosis of gastric carcinoma

XING Chengzhong, CHEN Junqing, XU Huimian.Department of Oncology, The First Affiliated Hospital, China Medical University, Shenyang110001

【Abstract 】 Objective Tostudy the change and significance of intracellular-free calcium levels ([Ca²⁺ ]i)and inositol 1, 4, 5-trisphosphate (IP₃ ) levels in the process of gastriccarcinoma cell apoptosis induced by cisplatin. Methods Apoptosisinduced by cisplatin in gastric carcinoma BGC823 cell was investigated by light and electronmicroscopy, agarose gel electrophoresis and flow cytometry. [Ca²⁺ ]iwas determined by Fura-2 fluorescin load technic, IP₃ was determined bycompetitive protein binding method. Results When BGC823 cells weretreated with cisplatin (2 μg/ml) for 24 hours changes appeared typical of apoptosis. [Ca²⁺ ]iand IP₃ were significantly increased, especially at the initial stage ofapoptosis. However, from 2 to 24 hours after cisplatin treatment, IP₃ levelsprogressively decreased, being significantly lower than those of the untreated controls. Conclusion Inthe process of cisplatin induced apoptosis of gastric carcinoma cells, [Ca²⁺ ]iand IP₃ levels are up-regulated at the very initial stage but down-regulatedfrom 2 hours afterwards.

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