冯涛 曾昭淳 周兰 陈文缘 左渝平 400046 重庆医科大学 中华肝脏病杂志 1998 0 0 3


    关键词：人肝特异性F抗原 酶联免疫吸附 肝病 期刊 zhgzbzz 0 临床研究 fur -->


    

【摘要】 目的 探讨血清人肝特异性F抗原作为肝脏功能指标在肝病诊断中的价值。方法 生物素-抗生物素蛋白酶联免疫吸附法(Bα-ELISA)测定各类肝病患者血清中人肝特异性F抗原。结果 Bα-ELISA方法灵敏、稳定。肝病血清F-Ag阳性率为85.2%(46/54)；对慢性肝炎、肝硬化和肝癌等的诊断价值高于天冬氨酸转氨酶(AST)；肝癌血清F-Ag的检出率为91.3%(21/23)，与甲胎蛋白含量具有相关性。结论 F-Ag可以作为判断肝损伤和肝脏功能状况的指标，对肝病尤其是肝癌的诊断具有明显的临床实用价值。

    

THE DETECTION OF SERUMHUMAN LIVER SPECIFIC F ANTIGEN AND PRELIMINARY CLINICAL APPLICATION

Feng Tao, Zeng Zhaochun, Zhou Lan, et al. Department of Biochemistry, Chongqing Medical University, Chongqing 400046

【Abstract】 Objectives To investigate the significance of F-Ag as thespecific marker of hepatocellular damage and the value in the diagnosis of liver disease. Methods A biotin-avidin ELISA(Bα-ELISA) was developed for themeasurement of human liver-specific F antigen(F-Ag) in sera of patients with various liverdiseases. Results Bα-ELISA werehighly sensitive. F-Ag was detectable in 46 of 54(85.2%) patients with various liverdiseases and 21 of 23(91.3%) hepatocellular carcinoma, showed a close correlation with ASTin acute hepatitis and AFP in hepatocellular carcinoma. Conclusion Theserum F-Ag concentration was a more sensitive and specific marker of liver damage thanconventional liver function tests. The use of F-Ag may facilitate detection ofhepatocellular carcinoma in some patients.

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