马为民 周伯平 王火生 徐六妹 袁静 付涌水 孙彤 518020 深圳市肝病研究所(马为民、周伯平、王火生、徐六妹、袁静)；广州市中心血站(付涌水)；中国科学院微生物研究所(孙彤) 中华肝脏病杂志 1998 0 0 3


    关键词：非甲～非庚型肝炎 输血传播病毒(TTV) 序列分析 基因型 职业献血员 静脉毒瘾者 期刊 zhgzbzz 0 临床研究 fur -->


    

【摘要】 目的 分析深圳地区不同人群中TTV(transfusion transmittedvirus, TTV)感染情况及其基因型。方法 在TTV ORF1设计引物，建立巢式聚合酶链反应，检测深圳地区不同人群血清中TTV DNA。对PCR产物进行分子克隆，以荧光法(Applied Biosystems, 373A)测序。结果 90例一般人群、88例有偿献血员、79例静脉毒瘾者、52例非甲～非庚型肝炎、67例乙型肝炎及72例丙型肝炎患者中，TTV DNA阳性率分别为7.8%、9.0%、41.8%、48.0%、22.3%与27.8%。5株深圳TTV(SZ1～SZ5)ORF1部分基因核苷酸序列同源性为66.2%～96.7%。基因分型5株中2株属G1型(G1a亚型)，3株属G2型，其中2株为G2b型。结论 深圳地区一般人群与职业献血员中TTV健康携带者较常见；静脉毒瘾者与非甲～非庚型肝炎患者是TTV感染的高危人群；乙型及丙型肝炎常重叠TTV感染；不同TTV分离株ORF1部分基因序列变异可达33%，基因分型以G1a与G2b为主。

    

MOLECULAR EPIDEMIOLOGICALSTUDY ON TTV INFECTION AMONG VARIOUS POPULATIONS IN SHENZHEN

Ma Weimin, Zhou Boping, Wang Huosheng, et al. Shenzhen Research Institute of Hepatology, Shenzhen 518020

【Abstract】 Objective To investigate transfusion transmitted virus(TTV) infection in various populations and sequence divergence among different TTVisolates. Methods A nested polymerase chain reaction (PCR) assay withprimers from ORF1 of TTV genome was establishied to detect TTV DNA in the serum samplesfrom various populations in Shenzhen. PCR product was cloned and sequenced with theApplied Biosystems(373A). Results TTV DNA was detected in the sera from 7of 90(7.8%) general individuals, 8 of 88(9.0%) paid blood donors, 33 of 79 (41.8%)intravenous drug users, 25 of 52(48.0%) patients with non A～non G hepatitis, 15 of 67(22.3%) patients with hepatitis B and 20of 72(27.8%) cases with hepatitis C. When partial sequence of ORF1 was compared among TTVisolates from 4 patients with non A～non G hepatitisand 1 blood donor, it showed divergence with differences 3.3% to 33%. Of the 5 sequences,2 belonged to genetic group G1 (subgroup G1a) and the remaining 3 to genetic group G2. Conclusion The results of the study suggest that TTV infection is common in the general populationand blood donors; TTV was detected frequently in patients with non A～non G hepatitis; the intravenous drug user is the population athigh risk for TTV infection and the sequence difference of a partial gene among differentTTV isolates may be up to 33%.

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