沈宁江 王书成 郑树森 沈宁江、王书成 570311海口,海南省人民医院骨病外科；郑树森 中山医科大学分析测试中心图像分析室 中华骨科杂志 1999 0 19 2


    关键词：电刺激；脊髓；引导组织再生 期刊 zhgkzz 0 脊柱脊髓疾患 fur -->


    

【摘要】目的 通过开展直流电场促进脊髓再生的实验研究，以证实其疗效并为临床提供依据。方法 32只中国家犬随机分成4组，用AllenWD法致脊髓完全损伤,立即置入电刺激器。电刺激器由小型高容量电池(12V、6V)、电阻、纯银丝、医用人体硅胶管等构成。A组为对照组，B组12V电刺激组，C组6V电刺激组，D组为脊髓损伤后6小时置入12V电刺激组。观察各组伤后1、2、3个月神经功能、皮层体感诱发电位、神经元数量、神经元截面积和尼氏体密度恢复情况。结果 电刺激组上述各项指标均优于同时期对照组(P<0.05或P<0.01),特别是神经功能，能早期、更好的恢复。伤后1个月,B组即能行走,C、D组可站起。此外,B组优于D组，差异有显著性意义。结论 直流电场确能有效地促进脊髓再生,但伤后6小时置入电刺激器的疗效比伤后立即置入电刺激器的疗效为差。

    Experimental Study ofUsing Direct Current Electric Field to Promote Spinal Cord Regeneration

SHEN Ningjiang ^* , WANG Shucheng, ZHENG Shusen.

    ^* Department of Orthopaedics, The People’s Hospital of Hainan Province, Haikou 570311

【 Abstract 】 Objective Toconfirm the effectiveness of using direct current electric field to promote spinal cordregeneration. Methods 32 Chinese dogs were randomly divided into fourgroups. After the complete spinal cord injury models were produced by Allen WD method, theelectric stimulators were applied immediately. The stimulator consisted of a miniaturehigh capacity battery (12V, 6V), resistance, pure silver wire, and silicon catheter. GroupA served as normal controls (0V), group B had 12V electric stimulator and group C 6V .Although group D had 12V , the stimulator was implanted into the body 6 hours after spinalcord injury. The nerve function, cortical somatosensory evoked potential and three kindsof morphometric changes i.e. the number of neurons, the cross section area of the neuronsand the recovery of the density of the Nissl’s bodies, were observed at 1 to 3 months after injury. Results All the above mentioned indexes in the electrical stimulated groups were much better thanthose in the control group (P<0.05 or P< 0.01). Especially, the neurologicalfunction recovery was much more rapid and better. At 1 month after striking, group A dogsstill had complete paraplegia, but group B could walk steadily, and group C, D couldstand. In addition, all indexes in group B were better than that in group D. Conclusion The direct current electric field can effectively promote spinal cord regeneration. But,the therapeutic effectiveness was much better when the stimulator was implanted earlierafter the injury.

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