赵西平 王宝菊 杨燕 杨东亮 郝连杰 430030 武汉，同济医科大学同济医院临床免疫研究室 中华肝脏病杂志 1998 0 0 2


    关键词：庚型肝炎病毒抗原 庚型肝炎病毒 RNA 免疫组织化学 期刊 zhgzbzz 0 临床研究 fur -->


    

【摘要】目的 探讨慢性肝病患者组织中庚型肝炎病毒(HGV)表达与意义。 方法 应用免疫组织化学方法以鼠抗HGV NS5单克隆抗体检测142例慢性肝病患者肝组织中HGV抗原，部分患者采用RT-PCR方法检测其血清中HGV RNA。 结果 142例肝病患者中，29例(20.4%)组织中检出HGV抗原，肝硬化组(42.9%, 12/28)较慢性肝炎(15.9%, 8/11)和肝癌(14.3%, 9/63)组检出率高。阳性信号位于胞浆中，阳性细胞可成散在、簇状或弥漫性分布。阳性细胞周围可有炎性坏死；肝癌患者抗原阳性细胞主要位于癌旁肝组织，癌巢中仅偶见少数散在分布的阳性细胞；绝大多数组织抗原阳性者其血清HGV RNA为阳性。有4例患者组织中HGV抗原阳性但其血清HGV RNA阴性。 结论 HGV可在慢性肝病包括肝细胞肝癌患者肝组织中表达，HGV感染在慢性肝炎病期进展及肝癌发生中具有一定意义。

    

IMMUNOHISTOCHEMICAL DETECTION AND SIGNIFICANCE OF HEPATITIS G VIRUS ANTIGEN IN LIVER TISSUE OF PATIENTS WITH CHRONIC LIVER DISEASES

Zhao Xiping, Wang Baoju, Yang Yan, et al. Clinical Immunology Research Unit, Tongji Hospital of Tongji Medical University, Wuhan, 430030

【Abstract】 Objective To study the expression and its significance of hepatitis G virus (HGV) in liver tissue. Methods HGV antigen was detected with immunohistochemical method using moloclonal mouse antibody to HGV NS5 in 142 patients with chronic liver disease, and serum HGV RNA was detected by RT-nested PCR in some of the subjects. Results Out of 142 subjects, 29(20.4%) had been detected to have HGV antigen in their liver, the detection rate in liver cirrhosis patients (12/28, 42.9%) was higher than that in chronic hepatitis (8/11, 15.9%) and hepatocellular carcinoma (HCC, 9/63, 14.3%) ones. The staining signal was located in liver cells cytoplasm, and the positive cells distributed scatterly, clusterly or diffusely. Necroinflammation could be observed around thd HGV antigen positive cells. In HCC, HGV antigen was mainly detected in tissues adjacent to cancerous cells, only a very few cancerous cells had been occasionally detected with the antigen. Most of the patients who had HGV antigen in their liver had been detected with HGV RNA in their serum except 4 cases who had been detected with HGV antigen in liver but without HGV RNA in their serum. Conclusion These results showed that HGV could express in the liver tissue of patients with chronic liver diseases including HCC, and HGV might have some role in the progression of chronic hepatitis and HCC formation.

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