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上海地区丙型肝炎病毒基因分型的研究
http://www.100md.com 《中华肝脏病杂志》 1999年第1期
丙型肝炎病毒基因型谱线探针分析,关键词:
     蒋伟伦 顾士民 胡芸文 许河生 孙亚洲 200083,上海市传染病医院(蒋伟伦,顾士民,胡芸文); 上海大江(集团)生物制药公司(许河生); 上海市公安局治安总院法医科(孙亚洲) 中华肝脏病杂志 1999 0 7 1


    关键词:丙型肝炎病毒 基因型 谱线探针分析 期刊 zhgzbzz 0 临床研究 fur -->


    

摘要 目的 研究上海地区不同人群中丙型肝炎病毒(HCV)基因型的分布和频率。方法 应用新改进的第二代谱线探针分析(Line Probe Assay,LiPA)试剂盒,对109份血清的HCVRNA逆转录-巢式PCR阳性分离物进行了基因分型。结果 81份丙型肝炎患者HCV分离物中71份(86.7%)为1b型,4份(4.9%)为2a/2c型,2份(2.5%)为3b型,1份(1.2%)为6a型,2份(2.5%)为混合型(2a/2c+2b+1和1b+6a),另有1份(1.2%)为未定型。7份献血员分离物中,5份为1b型,2份为2a型。但在21份静脉吸毒者分离物中,8份(38.1%)为1a型,5份(23.8%)为1b型,2份(9.5%)为2a/2c型,2份(9.5%)为3a型,1份(4.8%)3b型,3份(14.3%)为混合型(1a+2a/2c,1b+2a/2c+6a,1a+1b+3b各1份)。结论 (1)上海地区存在着分属4个HCV基因型(1、2、3和6)的7个亚型(1a、1b、2a/2c、2b、3a、3b和6a),其中2b、3a、3b和6a为首次发现。(2)HCV1b型最常见于丙型肝炎患者和献血员。1a和3a仅见于静脉吸毒者。(3)静脉吸毒者中存在较多的HCV基因型。

    

Gennotyping of HCV isolates fromdifferent populations in Shanghai by using second generation line probe assay

JIANG Weilun,GU Shimin,HU Yunwen,etal.Shanghai Municipal Infectious Disease Hospital,Shanghai 200083

Abstract Objective Toinvestigate the distribution and frequency of various genotypes in different populationsin Shanghai.Methods 109 HCV isolates by using RT-nested PCR including 81from patients with hepatitis C (PHCs),7 from blood donors (BDs) and 21 from introvenousdrug abusers (IVDAs) were genotyped by using recently improved second generation lineprobe assay (INNO-LIPA HCV Ⅱ innogenetics N.V.Belgium).Results Among 81HCV isolates from PHCs,there were 71 (87.6%) genotype1b,4(4.9%)2a,2(2.5%)3b,1(1.2%)6a,2(2.5%) mixed genotypes (1 for 2a or 2c+2b+1,1 for 1b+6a)and 1(1.2%) undeterminable respectively,and among 7 from BDs,there were 5 1b and 2genotype 2a.But among 21 from IVDAs,there were 8(38.1%) genotype 1a,5(23.8%)1b,2(9.5%)2aor 2c,2(9.5%)3a,1(4.8%)3b and 3(14.3%) mixed genotypes (1 for 1a+2a or 2c,1 for 1b+2a or2c+6a and 1 for 1a+1b+3b) respectively.Conclusion (1)7 HCV subtypes(1a,1b,2a,2b,3a,3b and 6a) belonging to 4 HCV genotypes (1,2,3,6) were present indifferent populations in Shanghai.Of them,2b,3a,3b and 6a were first found.(2)HCV genotype1b was most commonly found in PHCs and BDs.1a and 3a were only found in IVDAs.(3)The factthat more HCV subtypes were found in IVDAs in this city.

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