杨斌 赵敏 熊斌 刘珍君 刘元波 郭永刚 靳晓雷 北京，中国医学科学院中国协和医科大学整形外科医院 100041 中华整形烧伤外科杂志 1999 0 15 4


    关键词：贴附骨移植；骨计量学 期刊 zhzxsswkzz 0 论著 fur -->


    

【摘要】 目的 深入探讨骨贴附移植后的变化及不同胚胎来源骨质(膜状成骨和软骨成骨)移植后的差异。方法 应用新西兰兔、恒河猴采用荧光素双标记法，在不脱钙骨切片上，选用类骨质宽度、骨质矿化沉积率、荧光标记率等骨动力学指标对骨移植后的再生和改建进行计算机图像分析和定量测量。结果 骨体积存留率：膜状成骨为(78.4±3.5)%，软骨成骨为(56±5.1)%；骨矿化沉积率：膜状成骨为(3.60±0.8)μm/d，软骨成骨为(0.92±0.33)μm/d；类骨质宽度；膜状成骨为(14.56±2.69)μm，软骨成骨为(7.38±2.20)μm；荧光标记率：膜状成骨为(91.22±2.69)%，软骨成骨为(51.28±4.11)%。结论 膜状成骨移植后较软骨成骨可保持更多的骨质体积,有更为明显的成骨再生能力且可诱导宿主骨成骨的能力不同，膜状成骨优于软骨成骨。

Histological study and stereologic analysis of membranous versus endochondral onlay bone grafts

YANG Bin,ZHAO Min,XIONG Bin,et al.The Plastic Surgery Hospital of Chinese Academy of Medical Sciences, BaDaChu,Beijing 100041

【 Abstract 】 Objective The experimental study was to determine the differences in changes in the onlay bone grafts of different embryonic origin.Methods The study was designed to compare the potential for appositional bone growth in membranous and endochondral onlay grafts in New-Zealand rabbits and a rhesus monkey.Results were assessed with volumetric measurement,histological examinations and stereologic analysis with double-fluorochrome labeling technique.Results For the membranous and endochondral bone grafts,the volumetric retention rate was (78.4±3.5)% and (56.0±5.1)% respectively.The mineralization apposition rate (MAR) was (3.6±0.8) μ m/d and (0.92±0.33)μ m/d;the osteoid seam width (OSW) was (14.56±2.69) μ m and (7.38±2.20)μ m;the tetracycline uptake rate(TUA) was (91.22±2.69)% and (51.28±4.11)%,respectively.Conclusion ① It was found that the membranous bone graft maintained its volume to a significantly greater extent than the endochondral bone graft;② Membranous bone grafts exhibited higher survival rate and greater activity than endochondral bone grafts;③ Different changes were also found in the two recipient sites.There was more powerful bone regenerative reaction in the recipient bed of the membranous bone graft than the endochondral one.

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