王强 鲁开化 杨力 艾玉峰 蒋永培 710032 西安，第四军医大学西京医院整形外科中心(王强现在潍坊, 解放军第八十九医院，261021) 中华整形烧伤外科杂志 1999 0 15 2


    关键词：透明质酸刺激因子；成纤维细胞；透明质酸；嗜银蛋白；胶原 期刊 zhzxsswkzz 0 创面愈合 fur -->


    

【摘要】 目的 研究透明质酸刺激因子(HASF)对正常皮肤及瘢痕成纤维细胞活性和胶原合成的影响。方法 层析法从兔羊水及胎兔血清中提取HASF并做活性鉴定；用嗜银蛋白(AgNORs)染色法、³ H-脯氨酸掺入法和羟脯氨酸(HYP)测定法观察不同浓度的HASF对细胞增殖活性和胶原蛋白合成的影响。结果 HASF使两类细胞的AgNORs含量减少；两类细胞实验组脯氨酸掺入率与HYP含量均较对照组低，两种胶原测定法相关性好。结论 HASF对正常皮肤及瘢痕成纤维细胞的活性功能及胶原合成有抑制作用，可能影响伤口愈合中瘢痕的形成。

    Effects of hyaluronicacid-stimulating factor on viability and collagen synthesis of fibroblasts

WANG Qiang^* , LU Kaihua,YANG Li, et al.

    Plastic Surgery Genter, Xijing Hospital, Xi'an 710032

【 Abstract 】 Objective Tostudy the effects of hyaluronic acid-stimulating factor(HASF) on viability and collagensynthesis of fibroblasts derived from human dermis and scars. Methods HASFwas purified from amniotic fluid and fetal serum of gestational rabbits, and its activitywas determined. To observe the effects of HASF on viability and collagen synthesis of twocell lines, AgNORs staining, ³ H-proline incorporation and HYP determinationwere carried out.Results HASF reduces AgNORs contents in skin FB and scar FB; ³ H-proline incorporation and HYP contents were lower in experimental groupsthan those in controls in both cell lines. Two methods to detect collagen correlate wellwith each other.Conclusion HASF inhabits viability and collagen synthesisof both cell lines, thus may interefred with the formation of scar during wound healing.

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