【摘要】 目的 明确康宁克通和干扰素α-2b对增生性瘢痕成纤维细胞的抑制是否通过PDGF途径。方法 对6例增生性瘢痕同一个体分区域同时局部注射康宁克通或干扰素α-2b后3天及7天的PDGF BB mRNA原位表达进行了观察。结果 ①康宁克通或干扰素α-2b在局部注射后7天增生性瘢痕的PDGF BB mRNA表达强度比未注射区均明显减少(P<0.01)，但局部注射后3天表达强度与未注射区相比均无显著性差异(P>0.05)。②增生性瘢痕的PDGF BB mRNA原位表达强度高于正常皮肤。结论 激素及干扰素α-2b治疗瘢痕的机制之一是通过抑制PDGF基因表达，从而减少成纤维细胞的有丝分裂，使瘢痕的成纤细胞增殖受到抑制。

Effects of intralesional injection of kenalog or interferon α -2b on PDGF BB gene expression in situ of hypertrophic scars

XU Shaojun, BAO Weihan, YANG Xiaolin,et al.The Research Centre of Plastic Surgery,The Third Clinical School of Beijing Medical University,Beijing 100083

【 Abstract 】 Objective The study is to determine whether kenalog or interferon α -2b inhibits fibroblast growth in hypertrophic scars through decreasing PDGF.Method Six patients with hypertrophic scars received intralesional injection of kenalog or interferon α -2b.On the 3rd and 7th day after injection,scar samples from each patient were collected and PDGF mRNA expressions in situ were studied.Results ① On the 7th day after intralesional injection of kenalog or interferon α -2b,expressions of PDGF BB mRNAs decreased significantly (P<0.01).However,on the 3rd day the expressions of PDGF BB mRNAs of the intralesional-injected area and non-injected area were not significantly different (P>0.05).② Gene expressions of PDGF BB in situ were higher in hypertrophic scars than in normal skin.Conclusion The mechanism of kenalog or interferon α -2b in ameliorating hypertrophic scar is to reduce mitosis and prohibit proliferation of fibroblasts in hypertrophic scars by inhibiting PDGF gene expression.

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