唐洪泰 陈玉林 沈洪兴 李元义 白刚 葛绳德 陈士明 严小敏 夏照帆 唐洪泰 陈玉林 沈洪兴 李元义 白刚 葛绳德 夏照帆 上海，第二军医大学长海医院烧伤科 200433；陈士明 严小敏 复旦大学分析测试中心 中华整形烧伤外科杂志 1999 0 15 3


    关键词：烧伤；心肌细胞；细胞膜；顺磁共振；自旋标记 期刊 zhzxsswkzz 0 论 著 fur -->


    

【摘要】 目的 利用自旋标记—顺磁共振技术研究严重烧伤大鼠早期心肌细胞膜的变化。方法 用5-氮氧自由基硬脂酸(5-doxylstearic acid,5NS)和16-氮氧自由基硬酯酸((16-doxylstearic acid,16NS)分别掺入到不同深度的膜类脂区，观察严重烧伤对大鼠心肌细胞不同深度的膜类脂区流动性的影响，同时用3-甲基马来酰亚胺氮氧自由基(3-maleidoproxy1,3MP)标记膜蛋白巯基，观察膜蛋白旋转相关时间(τ_c )及强固定化与弱固定化组分谱线高度比值(h_s /h_w )的变化。结果 严重烧伤大鼠早期心肌细胞膜脂浅层的序参数(S)值无明显变化，而膜脂深层S值增大，膜蛋白h_s /h_w 显著增大，膜蛋白旋转相关时间τ_c 也显著增大。结论 严重烧伤后早期即出现心肌细胞膜的损伤，表现为膜脂质深层流动性下降，膜蛋白构象改变，膜蛋白运动受限。这些变化可以直接影响细胞膜蛋白的功能，从而导致心肌细胞功能的变化。

Early cardiac myocyte membrane changes in severely burned rats:a spin labding-ESR study

TANG Hongtai, CHEN Yulin, SHEN Hongxin, et al.Department of Burns,Changhai Hospital,Second Military Medical University,Shanghai,200433.

【 Abstract 】 Objective To demonstrate the mechanism of early cardiac myocyte membrane damage at molecular level in severely burned rats.Methods SD rats were randomly divided into sham-burn and burn groups.A 30% TBSA burn was created in burn group.Hearts were obtained at different time post-burn.Cardiac myocytes isolated by collagenase using Langendoff perfusion apparatus were spin labeled with 5-doxylstearic acid (5NS),16-doxylstearic acid (16NS) and 3-maleidoproxy1(3MP).The order parameters (S value),h_s /h_w ratio and rotational correlation time (τ _c ) were measured according to the spin labeling-ESR spectra.Results The S value of the polar head-group region of membrane lipid,as reported by 5NS,did not changed signicantly,while that of the lipid in the hydrophobic midzone,as reported by 16NS,increased signicantly.The h_s /h_w ratio of 2 and 6 hours postburn groups increased significantly postburn compared with that of the sham-burn group,and so did the rotational correlation time in 1 to 12h post-burn groups.Conclusion There were structural and functional changes in cardiac myocyte membrane after burn injury.They were characterized by decreased fluidity in the deeper part of membrane lipid,configurational changes and motion restriction in membrane proteins.They could affect the normal performance of cardiac function.

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