卓安山 陈爱娟 申繁星 罗丽玲 (卓安山、申繁星)132011 吉林市解放军第四六五医院；(陈爱娟)空军医学高等专科学校；(罗丽玲)中国医科大学附属二院 中华整形烧伤外科杂志 1999 0 15 2


    关键词：烧伤；免疫调节剂；免疫核糖核酸；巨噬细胞 期刊 zhzxsswkzz 0 论 著 fur -->


    

【摘要】 目的 探讨不同免疫调节剂对烫伤大鼠腹腔巨噬细胞(Mφ)功能的影响。方法 应用McAbAPAAP桥联酶标法、琼脂溶菌板法和MTT比色法等技术，分别测定了各组烫伤大鼠不同时相腹腔Mφ各种功能的变化。结果 ①烫伤后大鼠腹腔Mφ表面Ia抗原表达率降低，抗原提呈能力减弱，对念珠菌的噬菌率下降，溶菌酶活力减低，TNF分泌增高，且伤后第10天比第5天更明显。以上结果与正常组比较，差异有非常显著意义(P<0.01)。②经不同免疫调节剂治疗后，各组烫伤大鼠腹腔Mφ各种功能明显改善，各项指标与未用药的对照组比较，差异有非常显著意义(P<0.01)。结论 烫伤后早期腹腔注射特异性免疫核糖核酸(iRNA)可明显改善伤鼠的免疫功能。

    Effect of differentimmunomodulators on macrophage function in rats with scald injury

ZHUO Anshan^* , CHENAijuan, SHEN Fanxing, et al.

    The 465th Hospital of People's Liberation Army,Jilin,132011

【 Abstract 】 Objective Toexplore effect of different immunomodulators on macrophage (Mφ ) functions in rats with scald injury.Methods TheMφ functions invarious groups of rats with scald on the 5th and 10th day after scald were respectivelydetermined with methods of McAb APAAP, agar bacteriolytic plate and MTT colorimetry.Results The results showed that:① after scald,presenting rate of Ia antigen,ability of antigenpresentation,phagocytic power to candida albicans and lysozyme ability of Mφ were significantly decreased,thecapacity of Mφ secretingTNF was markedly increased compared with those of the normal group, and the differenceswere significant (P<0.01);② after the therapy with immunodulators,the above functions of Mφ in rats with scald improvedmarkedly.As compared with those of the control group,differences were significant(P<0.01).Conclusion The specific immune RNA,which is administered byintraperitoneal injection early after scald,can markedly improve immune functions of ratswith scald.

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