【摘要】目的 探讨烧伤后肾组织内皮素-1(ET-1)升高的效应部位以及效应的ETR亚型。方法 采用斑点杂交和原位杂交技术对大鼠30%体表面积Ⅲ度烧伤模型的ET-1及其受体亚型(ETA、ETB)的基因表达进行了研究。

     结果 烧伤后1小时，ET-1、ETA、ETB、mRNA均明显升高；烧伤后24小时仍维持较高水平。ET-1和ETA mRNA杂交信号于烧伤后3小时达高峰。ET-1mRNA主要分布肾皮质小血管内皮细胞、髓质肾小管和集合管，ETA受体mRNA则分布于上述小血管的平滑肌细胞。ETB受体mRNA于烧伤后6小时达高峰，主要分布髓质肾小管、集合管。

     结论 烧伤后肾脏内皮素受体亚型上调在皮质以ETA为主，在髓质以ETB为主，它们分别与增强表达的ET-1结合可能导致肾皮质缺血和水钠代谢异常，是烧伤后肾功能障碍的重要因素。

    Expression of mRNA for endothelin-1 and its receptors in rat kidney after burn injury Wu Xiongfei,Yang Zongcheng,Li Ao.Burn Research Institutes,The 1st Affiliated Hospital.The 3rd Military Medical University,

    Chongqing 400038.

     【Abstract】Objective and Methods We studied expression of messenger RNAS for endothelin-1(ET-1) and its receptor subunits (ETA,ETB) by northern blot hybridization and in situ hybridization in rat kidney following 30% TBSA full thickness burns. Results Expressions of that ET-1,ETA,and ETB mRNA expression are all evidently increased at 1 hour postburn.ET-1 and ETA reached its maximal mRNA expression at 3 hour postburn. ET\|1 mRNA distributed primarily on glomerular

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