刘亚光 尹培达 石成钢 赵湘茹 孔庆瑜 黄必军 刘亚光 尹培达 石成钢 孔庆瑜(510080 广州，中山医科大学附属第一医院风湿科)；赵湘茹(教学实验中心)；黄必军(肿瘤研究所) 中华皮肤科杂志 1999 0 32 1


    关键词：红斑狼疮；系统性；细胞死亡；Bcl-2 期刊 zhpfkzz 0 论著 fur -->


    

【摘要 】 目的 为了探讨bcl-2基因在系统性红斑狼疮(SLE)发病机制中的作用及临床意义。方法 应用逆转录-聚合酶链反应(RT-PCR)检测31例SLE患者外周血单一核细胞(PBMC)bcl-2 mRNA表达水平和流式细胞仪双标记法分析其T、B细胞bcl-2蛋白表达。结果 活动期SLE患者PBMC bcl-2 mRNA表达水平明显升高，占55.6%，且活动期SLE患者CD3+、CD4+和CD8+T细胞亚群bcl-2蛋白表达明显高于非活动期SLE患者、正常人对照组和其他疾病组，而CD19+B细胞bcl-2蛋白表达在各组之间并无统计学差异。结论 T细胞bcl-2异常表达在SLE疾病活动的过程中起着重要作用，检测SLE患者外周血T淋巴细胞bcl-2的表达水平可作为SLE的活动性指标。

Expression of bcl-2 in Lymphocytes from Patients with Systemic Lupus Erythematosus and Its Clinical Significance

LIU Yaguang, YIN Peida,SHI Chenggang,et al. Division of Rheumatology,Kidney Institute,First Affiliated Hospital, Sun Yat-Sen University of Medical Sciences,Guangzhou 510080

【Abstract 】 Objective To investigate the role of bcl-2 expression in the pathogenesis of systemic lupuserythematosus (SLE) and its clinical significance. Methods The bcl-2mRNA levels in peripheral blood mononuclear cells (PBMC) from 31 SLE patients were testedby reverse transcription-polymerase chain reaction (RT-PCR), and the bcl-2 protein levelsin T and B cells were also tested by two-colour cytofluorography. Results bcl-2 mRNA levels in PBMC increased significantly in 10 cases of active SLE, accountingfor 55.6% of active SLE patients. Compared with those in inactive SLE patients, normalcontrols and other non-SLE patients, the bcl-2 protein expression in T cell subsets(including CD3⁺ , CD4⁺ and CD8⁺ )increased significantly in active SLE patients. However, there was no statisticaldifference in bcl-2 protein levels in CD19⁺ B cells among allgroups. Conclusion The abnormal expression of apoptosis-related bcl-2in T cells might play an important role in disease activity of SLE , and the determinationof bcl-2 expression levels of peripheral T lymphocytes might be used as an index fordisease activity of SLE.

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