关键词：红斑狼疮；系统性；抗中性粒细胞胞浆抗体
【摘要 】 目的 为了探讨抗中性粒细胞胞浆抗体(ANCA)在系统性红斑狼疮(SLE)中的临床意义。方法 通过间接免疫荧光(IIF)、酶联免疫吸附试验和免疫印迹方法检测了84份SLE患者血清ANCA的阳性率和6种已知的靶抗原：蛋白酶3、髓过氧化物酶、杀菌-通透性增高蛋白、弹力蛋白酶、组蛋白酶G和乳铁蛋白。结果 间接免疫荧光法检测ANCA在SLE中的阳性率是22.6%，均为核周型ANCA；分别有44.1%和14.3%的SLE患者血清含有抗组蛋白酶G和乳铁蛋白自身抗体，且活动期的阳性率明显高于稳定期。结论 组蛋白酶G为ANCA的主要靶抗原，且抗组蛋白酶G抗体与SLE的病情活动有关。
Antineutrophil Cytoplasmic Antibodies and TheirTarget Antigens in Patients with Systemic Lupus Erythematosus
LUO Ting, ZHAO Minghui, MA Shengqing. Department ofDermatology, First Teaching Hospital, Beijing Medical University, Beijing 100034
【Abstract 】 Objective Toinvestigate the clinical significance of antineutrophil cytoplasmic antibodies(ANCA ) andtheir target antigens in systemic lupus erythematosus(SLE). Methods 84serum samples were collected,and indirect immunofluorescence(IIF) and ELISA were used todetect the positive rates of ANCA and 6 known target antigen, the 6 specific ANCA antigensincluded proteinase 3 (PR3), myeloperoxidase (MPO), bactericidal/permeability-increasingprotein(BPI), human leukocyte elastase (HLE), cathepsin G(CG) and lactoferrin(LF).Positive sera were furthur studied by Western blot analysis. The positive rate of ANCA wasalso compared between patients with (n=50) and without (n=34) lupus activities to revealwhether ANCA were correlated with lupus activities. Results 22.6%sera showed ANCA positive by IIF method, and all of them were peripheral nuclear ANCA.However, ELISA testing showed that 44.1% and 14.3% sera recognized CG and LF,respectively. The positive rates of anti-CG, and anti-LF antibodies in patients withactive lupus were significantly higher than those in non-active patients (P< 0.001). Conclusion Thetarget antigen of ANCA in SLE patients was CG, and anti-CG antibodies might be correlatedwith lupus activities.
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