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胶体金免疫层析法检测乙型肝炎表面抗原的评价
http://www.100md.com 《中华医学检验杂志》 1999年第6期
免疫酶技术  肝炎表面抗原,乙型  胶体金,关键词:
     曾章新 刘文星 朱忠勇 兰风华 350025 福州,南京军区福州总医院全军检验中心 中华医学检验杂志 1999 0 22 6


    关键词:免疫酶技术 肝炎表面抗原,乙型 胶体金 期刊 zhjyyxzz 0 论著 fur -->


    

摘要 】 目的 对4种胶体金免疫层析测定(GICA)试条检测乙型肝炎表面抗原(HBsAg)的性能、结果等技术参数进行评价,为各实验室的选用提供参考。方法 采用GICA法和酶免疫(EIA)法对定值的HBsAg样品和2158份全血或血清标本同时测定,并结合有关物理现象作观察,对4种GICA试条的敏感性、特异性、试条渗透性、固相膜均匀性等结果进行评价。结果 4种GICA试条对HBsAg的最小检出量为1~5 ng/ml;与EIA法比较,检出率为95.6%~98.7%,一般不出现假阳性,未发现前带反应;检测所需时间5~30分钟,但试剂成本为EIA法的4~5倍。结论 GICA适用于急诊检验,便于献血员现场采血前的筛选;其敏感性低于EIA法,成本高;单独用于献血员筛选并不可靠,必须与EIA法并用,实行双检。

Gold immunochromatographyfor HBsAg detection

    
ZENG Zhangxin, LIU Wenxing, ZHU Zhongyong, et al.

    PLA Center for Laboratory Medicine, Fuzhou General Hospital, Fuzhou 350025

Abstract Objectives To evaluate the quality andperformance of 4 sources of gold immunochromatography assay (GICA) strips for HBsAgdetection and to provide data for various laboratories in the selection of HBsAg assaymethods. Methods Severalknown sera and 2158 unknown sera were tested with both GICA strips and EIA. Thesensitivity, specificity and physical properties of the strips were assessed. Results The minimal detection limits ofthe 4 sources of GICA strips were 1-5 ng/ml, with detection rates varying from 95.6 to98.7%, as compared with EIA. No false positives and pre-band phenomena were found. GICAneeded 5 to 30 min to complete. It costed 4 or 5 times as much as EIA. Conclusions GICA is fit for emergency andrapid screening of blood donors. It is less sensitive than EIA, and costs more. In blooddonor screening, GICA should be combined with EIA, since screening only by GICA may not bereliable.

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