陈国安 孙燕 李申德 刘天菊 杨德昌 710061 西安医科大学(陈国安、刘天菊、杨德昌)；中国医学科学院中国协和医科大学肿瘤医院(孙燕、李申德) 中华结核和呼吸感染 1999 0 22 3


    关键词：肺肿瘤；脱氧核糖核酸；聚合酶链反应 期刊 zhjhhhxgr 0 论 著 fur -->


    

【摘要】 目的 研究微卫星DNA在肺癌组织中的改变以及其对肺癌的诊断价值。方法 应用聚合酶链反应(PCR)-银染法，对50例原发性肺癌及自身正常肺组织3P13-26上的5个微卫星位点进行检测。结果 50例肺癌中，微卫星不稳定发生率为52%(26/50)，杂合性丢失38%(19/50);低分化肺癌微卫星不稳定发生率(77%)明显高于高、中分化(36%);微卫星不稳定联合杂合性丢失对肺癌的诊断阳性率达72%。结论 微卫星不稳定和杂合性丢失在肺癌发生、发展中可能起一定作用。在肺癌诊断上可能有一定价值。

    The alteration of microsatellite DNA in lung cancer CHEN Guoan^* ,SUN Yan, LI Shende， ^* XI′an Medical University, Xi′an 710061

    【Abstract】 Objective To study the alteration ofmicrosatellite DNA in primary lung cancer and its value of diagnosis for lung cancer. Methods Fivemicrosatellite markers on chromosome 3P13-26 have been examined in 50 primary lung cancertissues and their normal lung tissues. Results In fifty lung cancer,theincidence of microsatellite instability(MSI) was 52%(26/50) and the loss of heterozygosity (LOH) 38%(19/50). The positive rate of MSI in poorly differentiated lungcancer(13/17,77%)was significantly higher than that in well,moderatelydifferentiated(10/28, 36%). The sensitivity in the diagnosis of lung cancer was 72% bycombining MSI and LOH. Conclusions MSI and LOH may play a certain rolein the carcinogenesis and progression of lung cancer and they may be valuable molecularmarkers in diagnosis of lung cancer.

     ......

您现在查看是摘要页，全文长 12202 字符。