【摘要】 目的 探讨Amplisensor-聚合酶链反应(AmpliSensor-PCR)在结核病诊断中的价值。方法 采用AmpliSensor-PCR对784例结核病患者及160例肺癌患者的标本进行检测，并与PCR(凝胶电泳后，经溴化乙锭染色)、涂片、培养等法比较。结果 AmpliSensor-PCR的敏感性显著高于涂片及培养(P<0.01)。特异性较PCR法高。结论 AmpliSensor-PCR可以通过标准曲线划定检出下限，并可换算出标本中原始的靶DNA值，同时具有较高的特异性和敏感性，对肺结核尤其是肺外结核的诊断有一定的临床意义。

    Detection of Mycobacterium tuberculosis by AmpliSensor-PCRtechnique and its clinical application

    Tan Yaoju, Li Yigeng, Tan Shouyong, et al. Pulmonary Disease Institute, Guangzhou ChestHospital, Guangzhou 510095

    【Abstract】 Objective To explore thevalue of Amplisensor-PCR method in diagnosis of tuberculosis. Method The samples from 784 patients with tuberculosis, 160 patients withlung cancer were detected by using AmpliSensor-PCR, PCR, smear and culture methods, andthe results were compared. Result The sensitivity ofAmpliSensor-PCR technique was obviously higher than that of smear and culture (P<0.01).The specificity of AmpliSensor-PCR method was higher than that of PCR method. Conclusion Amplisensor-PCR method can be determined to detect the lower limitby standard curve, through which the original amount of target-DNA in samples can becalculated. AmpliSensor-PCR method shows higher specificity and sensitivity. It is ofclinical value in diagnosis of pulmonary tuberculosis and particularly of extrapulmonarytuberculosis.

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