李彪、朱承谟、杨卫东、张圣国、张冀先：200025 上海第二医科大学附属瑞金医院核医学科；吴祥甫：中国科学院上海生物化学研究所 中华核医学杂志 1999 0 19 3


    关键词：抗体，单链；癌胚抗原；结肠直肠肿瘤；放射免疫显像；动物，实验 期刊 zhhyxzz 0 论著 fur -->


    

摘要 目的 研究¹²⁵ I、¹³¹ I标记抗癌胚抗原单链抗体(CEAScFv)在荷人结直肠癌裸鼠体内的分布和定位显像。方法 采用Iodogen法制备¹²⁵ I-CEAScFv和¹³¹ I-CEA ScFv。20只荷人结直肠癌裸鼠，腹腔注射¹²⁵ I-CEAScFv后1、3、6和24 h进行裸鼠的体内分布研究,对3只荷人结直肠癌裸鼠腹腔注射¹³¹ I-CEAScFv后1、3、6和24 h进行裸鼠的定位显像研究。结果 在荷人结直肠癌裸鼠腹腔注射¹²⁵ I-CEAScFv后3 h，肿瘤与血之比为1.28，6 h达5.50，24 h达到最高，为6.46。腹腔注射¹³¹ I-CEAScFv后，6 h肿瘤部位放射性明显浓聚，24 h本底明显降低，肿瘤呈放射性热区。结论 CEAScFv能提高靶/非靶组织比值，缩短显像时间，改善图像质量。

The biodistribution andradioimmunoimaging of anti-CEA single-chain antibody in human rectocolonic tumor bearingnude mice

LI Biao,ZHU Chengmo,YANG Weidong,etal.Ruijin hospital,Shanghai No.2 Medical University,Shanghai 200025

Abstract Objective Toinvestigate the biodistribution and the radioimmunoimaging of ¹²⁵ I,¹³¹ Ilabeled anti-CEA single-chain antibody (¹²⁵ I-CEA ScFv and ¹³¹ I-CEAScFv) in human rectocolonic tumor bearing nude mice.Methods The CEAScFv was radioiodinated (¹²⁵ I,¹³¹ I) by iodogen solid labellingmethod.The uptake of radioactivity in tumor and normal tissues in 20 human rectocolonictumor bearing nude mice was measured at 1,3,6 and 24 hour after peritoneal injection of ¹²⁵ I-CEAScFv and in 3 human rectocolonic tumor bearing nude mice imaging was carried out at 1,3,6and 24 hour after peritoneal injection of ¹³¹ I-CEA ScFv.Results Thetumor to nontumor tissue ratio (T/NT,tumor to blood) reached 1.28,5.50 and 6.46 at 3,6 and24 h respectively.Radioactivity markedly concentrated at 6 h in tumor site anddemonstrated a “hot” area with significant decreasing its background at 24 h.Conclusions CEAScFv can elevate the T/NT ratio,shortening imaging interval and improve image quality.

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