傅小兵 杨银辉 孙同柱 王亚平 郭振荣 盛志勇 解放军第304医院(北京，100037) 中国修复重建外科杂志 1999 0 13 5


    关键词：糖尿病溃疡；转化生长因子β1；白细胞介素6；逆转录多聚酶链反应 期刊 zgxfcjwkzz 0 生长因子的研究与应用 fur --> /info/meeting/meeting02.asp?keyword=整形外科学


    

【摘 要】 目的 研究转化生长因子β₁ (TGF-β₁ )与白细胞介素6(IL-6)mRNA在糖尿病溃疡创面组织中的变化规律以及与组织修复结局的关系。方法 采用逆转录PCR(RT-PCR)技术和病理学方法，研究TGF-β₁ 和IL-6两种生长因子mRNA在糖尿病患者正常皮肤与溃疡创面的不同表达。结果 在模板量相同以及反应条件一致的情况下，溃疡创面组织TGF-β₁ mRNA的表达量显著降低，而IL-6 mRNA表达则较正常对照明显增加。结论 创面内源性TGF-β₁ 和IL-6mRNA表达的不同，以及两种因子产生减少或活性降低，是使组织修复能力下降，最终导致慢性创面长期不愈合的原因之一。

TRANSFORMING GROWTH FACTORβ ₁ ANDINTERLEUKIN 6 mRNA EXPRESSION IN WOUND TISSUES OF PATIENTS WITH DIABETIC ULCERS

FU Xiao-bing,YANG Yin-hui,SUNTong-zhu,et al.

The 304th Hospital of PLA.Beijing,P.R.China 100037

【 Abstract】 Objective To study the relationship between the changes of mRNAexpression in wound tissues of diabetic ulcers and tissue repair.Methods The mRNA expression of TGF-β ₁ and IL-6 in eightbioptic samples of diabetic ulcers were detected by RT-PCR and pathologic methods, and thesurrounding normal skins from the same patients were measured as control group. Results The mRNA expression levels ofTGF-β ₁ weremarkedly decreased in the diabetic ulcers compared with control group, while the mRNAexpression levels of IL-6 were increased at the same reaction conditions.Conclusion The different changes of mRNAexpression level of TGF-β ₁ and IL-6 in wound tissue result in low production anddecreased activity of TGF-β ₁ and IL-6,which lower the reparative ability of woundtissue.

     ......