洪帮兴 江丽芳 张欣 郭辉玉 洪帮兴(中山医科大学微生物学教研室，广州510089)；江丽芳 张欣 郭辉玉(广东省深圳市福田卫生防疫站，深圳518033) 中国免疫学杂志 2000 0 16 12


    关键词：登革病毒；基因免疫；免疫应答 期刊 zgmyxzz 0 641-643 分子与细胞免疫学 fur -->


    

摘 要 目的：研究登革病毒E基因免疫的可行性。方法：用脂质体转染法将构建的E基因重组真核表达质粒pcDNA3-E导入小鼠成纤维细胞NIH3T3细胞，SDS-PAGE和蛋白质印迹试验检测E基因的体外表达。然后将该重组真核表达质粒经肌肉注射免疫BALB/c小鼠，检测其诱发特异性免疫应答水平。结果：重组真核表达质粒pcDNA3-E在小鼠体内诱发一定水平的体液和细胞免疫应答，且持续时间较长。结论：登革病毒E基因免疫可诱发特异性免疫应答，为登革病毒疫苗的研制提供了实验依据。

    中国图书分类号 R373.33

Immunogenicity of dengue virus E antigen gene in BALB/c mice

HONG Bang-Xing JIANGLi-Fang ZHANG Xin

    (Department of Microbiology,Sun Yat-sen University of Medical Sciences,Guangzhou 510089)

Abstract Objective:Tostudy the possibility of dengue virus E gene vaccine.Methods:The recombinant eukaryoticexpression plasmid pcDNA3-E was first transfected into NIH3T3 cells by lipofectin.SDS-PAGEand Western blotting analyzed the expression of E gene.Then the recombinant plasmid wasintramuscularly injected to BALB/c mice,and the specific humoral and cellular immunitywere tested.Results:The recombinant plasmid DNA could induce specific immune reactions andthe immune response could last a long time.Conclusion:The dengue virus E gene vaccinecould induce specific immune reaction,which might have provided some material and newexperimential data for the further study of dengue vaccines.

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