倪卫东 安 洪 蒋电明 曹本珍 胡永康 重庆医科大学附属第一医院骨科(重庆，400016) 中国修复重建外科杂志 1999 0 13 5


    关键词：骨移植；脱蛋白骨；骨形态发生蛋白；生长因子 期刊 zgxfcjwkzz 0 生长因子的研究与应用 fur -->


    

【摘 要】 目的 比较三种重组合人工骨的成骨能力。方法 在50只兔的100个颅骨缺损动物模型中分别植入：①牛脱蛋白骨(bDPB)/牛骨形态发生蛋白(bBMP)/肿瘤坏死因子α(TNFα)；②bDPB/bBMP/碱性成纤维细胞生长因子(bFGF)；③bDPB/bBMP/表皮细胞生长因子(EGF)；④bDPB/bBMP。植入后1、2、4、6及8周行组织学检查及X线摄片；植入后10及42天行 ³⁵ S和⁴⁵ Ca液闪计数及灰重测定。结果 成骨能力：bDPB/bBMP/TNFα>bDPB/bBMP/bFGF(P<0.01)>bDPB/bBMP/EGF(P<0.01)；bDPB/bBMP/EGF与bDPB/bBMP无显著性差异(P>0.05)。结论 TNFα与BMP及载体复合后，在体内对骨修复产生明显的促进作用，在载体/骨诱导因子/生长因子的重组合人工骨模式中，DPB/bBMP/TNFα是一种有价值的骨移植材料。

COMPARATIVE STUDY ONOSTEOGENESIS OF THREE TYPES GROWTH FACTORS RECOMBINATION ARTIFICIAL BONES

NI Wei-dong,AN Hong,JIANGDian-ming, et al.

Department of Orthopedic Surgery,First Affiliated Hospital, Chongqing University of Medical Science.Chongqing, P.R. China400016

【 Abstract】 Objective To compare the osteogenesis of recombination artificialbones, which are bovine deproteined bone (bDPB) and bovine bone morphogenetic protein (bBMP), combined with tumor necrosis factor α (TNFα ),basic fibroblast growth factor (bFGF), epidermal growth factor (EGF) respectively.Methods One hundred trephined skull bone defects in fifty rabbits weredivided into four groups, which implanted with bDPB/bBMP/TNFα ,bDPB/bBMP/bFGF, bDPB/bBMP/EGF, and bDPB/bBMP respectively.X-ray and histological changes were observed in the 1st, 2nd, 4th, 6th, 8thweeks after implantation.The content of ³⁵ S and ⁴⁵ Ca and ash weight were measured at 10 and 42 daysafter operation.Results The osteogenesis of bDPB/bBMP/TNFα group was stronger than that of bDPB/bBMP/bFGF group(P< 0.01), while bDPB/bBMP/bFGF groupwas stronger than that of bDPB/bBMP/EGF(P< 0.01).No significant statistical difference were found between bDPB/bBMP/EGF and bDPB/bBMP(P> 0.05).Conclusion TNFα combined with bBMP and carrier can stimulate bone formation andincrease the volume of new bone in vivo.It suggests that bDPB/bBMP/TNFα is a valuable biomaterial of bonegraft.

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