关键词:17β-雌二醇;动作电位;L型钙电流;豚鼠;心室乳头肌;心室肌细胞
摘要 探讨雌激素对心血管保护作用的机制,采用标准玻璃微电极技术及全细胞膜片钳技术观察17β-雌二醇(ES)对豚鼠心室乳头肌细胞动作电位的影响及其对分离的豚鼠心室肌细胞的L型钙通道电流的作用。结果:30 μmol/L ES灌流心肌30 min可明显缩短动作电位时程;复极20%动作电位时程及复极50%动作电位时程分别由146±22 ms,213±21 ms缩短至87±6 ms,107±11 ms(P均<0.01)。1,10,20,30 μmol/L ES呈浓度依赖性抑制L型钙通道电流(P均<0.01),抑制率分别为8%,18%,33%,43%。结论:ES是一种内源性的钙离子通道拮抗剂,这可能是其心血管保护作用的重要机制之一。
Effects of 17β -Estradiol on Action Potential and L-Type Calcium Current
in Guinea Pig Ventricular Myocytes
Wang Jiyun,Hu Dayi,Liu Bo,et al
(Heart Center,Beijing Red Cross Chaoyang Hospital,Beijing,100020)
Abstract To investigate the protective mechanism of estrogen on cardiovascular diseases,standard glass microelectrode method and whole cell patch clamp technique were used to study the effects of 17β -estradiol on action potential of guinea pig ventricular papillary muscle and L-type calcium current of ventricular myocytes.Results:ES 30 μ mol/L shortened action potential duration (APD),especially 20% of APD and 50% of APD ......
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