欧阳彬 王吉甫 510080 广州，中山医科大学附属第一医院普通外科 中国胃肠外科杂志 1999 0 2 3


    关键词：一氧化氮；脓毒血症；存活率；三磷酸腺苷；线粒体 期刊 zgwcwkzz 0 论 著 fur -->


    

【摘要 】 目的 观察诱导型一氧化氮合酶(NOS)抑制剂L-N⁶ -亚氨乙基-赖氨酸(L-NIL)对脓毒血症小鼠细胞能量代谢和存活率的影响。方法 制作小鼠盲肠结扎穿孔(CLP)动物模型，分别注射0.9%氯化钠溶液或L-NIL或L-NIL加L-精氨酸(L-Arg)，比较各组小鼠肝细胞线粒体结构、心肌ATP、ADP含量和小鼠存活情况。结果 L-NIL使CLP模型小鼠肝细胞线粒体结构损伤减轻，心肌细胞内ATP含量增加，ATP/ADP比例增加，小鼠18小时存活率由20%增加至60%。结论 诱导型NOS抑制剂L-NIL能改善脓毒血症小鼠细胞能量代谢及提高其存活率。

Effect of L-NIL on cellular energy metabolism and survival rate in sepsis mice

OUYANG Bin， WANG Jifu. Department of Surgery,First Affiliated Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510080

【Abstract 】 Objective To observe the effect of inducible nitric oxide synthase inhibitor L-NIL (L-N⁶ -iminoethyl-lysine) on cellular energy metabolism and survival rate in cecum ligation puncture (CLP) model. Methods 0.9% natrii sodium or L-NIL or L-NIL + L-Arg was injected into CLP mice respectively. The mitochondrial structure of liver cell, the content of ATP and the ratio of ATP/ADP of cardiomyocyte and the survival rate of mice were compared between groups. Results The damage of mitochondrial structure was attenuated, the content of ATP and the ratio of ATP/ADP of cardiomyocyte was increased ......