孙 备 姜洪池 乔海泉 孙金圣 朱世军 王孝铭 孙 备 姜洪池 乔海泉 哈尔滨医科大学第一临床医学院普外科(哈尔滨 150001)；孙金圣 朱世军 王孝铭 哈尔滨医科大学病理生理教研室 中国普外基础与临床杂志 1999 0 0 4


    关键词：保存；内皮素-1；大鼠；肝脏 期刊 zgpwjcylczz 0 基础与实验研究 fur -->


    

摘要 为探讨改进大鼠肝脏低温保存方法对鼠肝的影响，即将切取后的鼠肝灌入一定量的自制保存液(HYD液)后结扎进出肝脏的各血管。应用大鼠离体肝灌注模型，对传统保存法(对照组)和改进保存法(20%组、30%组及40%组)肝脏微循环指标(门静脉灌注压、流出液内皮素-1、台盼蓝分布时间及组织学改变)、流出液有关酶指标水平及分泌胆汁量进行了观察。结果：20%组、30%组及40%组肝脏微循环指标及分泌胆汁量明显优于对照组(P<0.05)，30%组肝脏有关酶指标水平明显低于对照组(P<0.05)，30%组的保存肝脏效果最佳。本实验结果提示：改进保存法保存鼠肝的各项指标明显优于传统保存法，故本法行之有效，值得临床工作借鉴。

    

STUDY ON MODIFIED COLD STORAGE METHOD OF RATLIVERS WITH SELF-MADE HYD SOLUTION

Sun Bei, Jiang Hongchi, Qiao Haiquan, et al. Department of General Surgery, First Clinical Hospital, Ha’erbin Medical University, Ha’erbin 150001

Abstract To investigate the effectof modified cold preservation on rat livers. The modified method was that the vascular bedof rat liver was infused with an additional 20 ml, 30 ml and 40 ml self-made HYDsolution/100g liver. Via portal vein and the vesselses of liver were ligated. Forty ratswere randomly divided into four groups including conventional storage group (controlgroup) and modified preservation groups (20%, 30% and 40% group). The indices of hepaticmicrocirculation, enzymes level in the effluent and bile production were determined in thegroups. Results: Bile production and all the indices of hepatic microcirculation includingportal perfused pressure, endothelin-1 in the effluent, Trypan Blue distrubution time andhistology in experimental groups were significantly superior to those in control group (P<0.05).The liver enzymes in 30% group were markedly lower than those in control group (P<0.05).The preservation effect of rat livers in 30% group was the best among the experimentalgroups. Conclusions: The modified cold storage method is effective and may have potentialfor clinical application for liver preservation.

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