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肝细胞癌组织和外周血中肿瘤/睾丸抗原SSX-2和SSX-5的表达
http://www.100md.com 吴力群, 王新建, 张 斌, 卢 云, 杨金镛
肝细胞癌;SSX-2;SSX-5;逆转录聚合酶链反应,吴力群,王新建,张斌,卢云,杨金镛,通讯作者,:,Expressionofc
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     吴力群, 王新建, 张斌, 卢云, 杨金镛 , 青岛大学医学院附属医院肝胆外科 山东省青岛市 266003

    吴力群, 男, 1958-10-31生, 江苏省丹阳市人, 汉族, 1982年青岛医学院本科毕业, 主任医师, 主要从事肝胆外科专业.

    通讯作者 : 吴力群, 266003, 山东省青岛市, 青岛大学医学院附属医院肝胆外科. jiaoqin@mail.qdcatv.com.cn

    电话: 0532-82911369 传真: 0532-2911999

    收稿日期: 2005-03-31 接受日期: 2005-04-09

    Expression of cancer-testis antigen SSX-2 and SSX-5 in tissues and peripheral blood of patients with hepatocellular carcinoma

    Li-Qun Wu, Xin-Jian Wang, Bin Zhang, Yun Lu, Jin-Yong Yang

    Li-Qun Wu, Xin-Jian Wang, Bin Zhang, Yun Lu, Jin-Yong Yang, Department of Hepatobiliary Surgery, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China

    Correspondence to: Li-Qun Wu, Department of Hepatobiliary Surgery, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China. jiaoqin@mail.qdcatv.com.cn

    Received: 2005-03-31 Accepted: 2005-04-09

    Abstract

    AIM: To investigate the expression of SSX-2 and SSX-5 mRNA in the tissues and peripheral blood of patients with hepatocellular carcinoma (HCC) and their relations with kinds of cilinical indexes, and to evaluate the possibility of using them as the targets for specific immunotherapy of HCC as well as the markers to in adjuvant diagnosis and prognosis and recurrence detection of HCC.

    METHODS: One-step reverse transcription polymerase chain reaction (RT-PCR) wasused to detect the expression of SSX-2 and SSX-5 mRNA in the cancer tissues, corresponding cancer-adjacent tissues and peripheral blood of 26 patiernts with HCC, as well as in12 cirrositic tissues and 10 normal tissuesOne sample, selected from each gene with positive PCR outcomes, was sequenced to testify the reliability. The relation between SSX-2, SSX-5 expression and kinds of clinical indexes, such as a-fetoprotein (AFP), HBsAg, diameter of the tumor, TNM staging, was analysed.

    RESULTS: The positive rates of SSX-2 and SSX-5 mRNA expression were 34.6%(9/26) and 46.2%(12/26) in HCC tissues respectively, while none of the two genes was detected in the corresponding cancer-adjacent tissues. At least one gene was expressed in 65.4%(17/26) of HCC tissues and the co-expression was found in 15.4%(4/26). In the peripheral blood of the 26 HCC patients, the positive-expression rates of SSX-2 and SSX-5 mRNA were 19.2%(5/26) and 23.1%(6/26) respectively. The positive rates of single expression and co-expression were 34.6%(9/26) and 7.7%(2/26) respectively. In the 12 patients with hepatic cirrhosis and 10 nomal controls, none of SSX-2 and SSX-5 mRNA was detectable in liver tissues and peripheral blood. After sequencing, the RT-PCR products were confirmed to be the target cDNA. No relationship was found between the expression of the two genes and the clinical indicators such as age, sex, tumor size, serum AFP level and infection of hepatitis B virus (P>0 ......

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