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三氧化二砷注射液对胰腺癌细胞系PC-3的体外作用
http://www.100md.com 刘静冰, 秦叔逵, 李 进
三氧化二砷;胰腺癌;MTT法;流式细胞术;免疫组化,刘静冰,秦叔逵,李进,通讯作者,:,Effectofarsenictrioxideonpa
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     刘静冰, 秦叔逵 , 中国人民解放军第八一医院全军肿瘤中心

    江苏省南京市 210002

    李进 , 上海复旦大学医学院附属肿瘤医院 上海市 200032

    刘静冰, 女, 1975-04-06生, 江苏省睢宁县人, 汉族, 2001级南京中医药大学中西医结合肿瘤内科硕士.

    南京军区医学科研“十·五”计划课题, No. 02MA014

    通讯作者 : 秦叔逵, 210002, 江苏省南京市杨公井34标34号, 中国人民解放军第八一医院全军肿瘤中心肿瘤内科. qinsk@csco.org.cn

    电话: 025-84453932 传真: 025-84453906

    收稿日期: 2005-02-14 接受日期: 2005-03-22

    Effect of arsenic trioxide on pancreatic carcinoma cell line PC-3 in vitro

    Jing-Bing Liu, Shu-Kui Qin, Jin Li

    Jing-Bing Liu, Shu-Kui Qin, Oncology Center of Chinese PLA 81st Hospital, Nangjing 210002, Jiangsu Province, China

    Jin Li, the Affiliated Tumor Hospital, Medical College, Shanghai Fudan University, Shanghai, 200032, China

    Supported by Nanjing Military Region, No. 02MA014

    Correspondence to: Shu-Kui Qin, Cancer Center of PLA 81st Hospital, No.34, 34 Biao, Yanggongjing Street, Nanjing 210002, Jiangsu Province, China. qinsk@hotmail.com

    Received: 2005-02-14 Accepted: 2005-03-22

    Abstract

    AIM: To explore the biological effect of arsenic trioxide (As2O3) on human pancreatic carcinoma cell line PC-3 and its mechanism.

    METHODS: MTT assay was used to observe the inhibitory actions of As2O3 on PC-3 cells at various concentrations. The apoptotic rate and growth cycle of the cells were detected by flow cytometry. The changes of the cells ultrastructures were observed under electron microscope. The expression of apoptosis-related gene protein (Fas, Fas-L, Bcl-2, Bax) was detected by immunohistochemical staining.

    RESULTS: As2O3 inhibited the proliferation of PC-3 cells in a concentration- and time-dependent manner. Marked apoptosis peak was observed and the cells were mainly blocked in S phase (14.86-63.66%). PC-3 cells showed obvious feature of apoptosis under electron microscope, such as intact cell membrane, pyknosis of chromatin, nuclear fragmentation and apoptotic body formation. Bcl-2 protein was weakly expressed (+++) in controls, but strongly expressed (+) in As2O3-treated cells. Fas-L protein were weakly (+) and negatively (-) expressed in controls respectively, but strongly expressed (+++) in As2O3-treated cells.

    CONCLUSION: As2O3 can inhibit the proliferation and induce the apoptosis of human pancreatic carcinoma cell PC-3 and the mechanism is probably related to its effect on the regulation of Fas, Fas-L and Bcl-2 expression.

    Key Words: Arsenic trioxide; Pancreatic carcinoma; MTT assay; Flow cytometry; Immunohistochemistry

    Liu JB, Qin SK, Li J. Effect of arsenic trioxide on pancreatic carcinoma cell line PC-3 in vitro. Shijie Huaren Xiaohua Zazhi 2005;13(14):1713-1716

    摘要

    目的 : 探讨三氧化二砷(亚砷酸,As2O3)注射液对人类胰腺癌细胞的生物学效应及其作用机制 ......

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