重组人内皮抑素真核表达载体pCD-sEndo的构建和表达
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邵俊伟, 刘然义, 易继林, 卢绮萍, 黄文林
内皮抑素;重组真核表达载体;肝癌,邵俊伟,卢绮萍,刘然义,黄文林,易继林,通讯作者,:,Constructionofrecombinan
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邵俊伟, 卢绮萍, 广州军区武汉总医院普通外科 湖北省武汉市 430070
刘然义, 黄文林, 中山大学肿瘤研究所 广东省广州市 510060
易继林, 华中科技大学同济医学院附属同济医院普通外科
湖北省武汉市 430030
邵俊伟, 男, 1970-01-15生, 湖北省孝感市人, 汉族, 2004年华中科技大学同济医学院医学博士毕业, 主治医师, 主要从事肝胆外科治疗和基础研究.
通讯作者: 邵俊伟, 430070, 湖北省武汉市武昌珞狮路76号书香门第801, 广州军区武汉总医院普通外科. sjwsjw555555@yahoo.com
电话: 027-87164789
收稿日期: 2005-05-19 接受日期: 2005-06-08
Construction of recombinant eukaryotic expression vector for human endostatin and its expression in liver cancer cell line SMMC-7721
Jun-Wei Shao, Ran-Yi Liu, Ji-Lin Yi, Ji-Ping Lu, Wen-Lin Huang
Jun-Wei Shao, Ji-Ping Lu, Department of General Surgery, Wuhan General Hospital, Guangzhou Military Command of Chinese PLA, Wuhan 430070, Hubei Province, China
Ran-Yi Liu, Wen-Lin Huang, Institute of Oncology, Zhongshan University, Guangzhou 510060, Guangdong Province, China
Ji-Lin Yi, Department of General Surgery, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Correspondence to: Dr. Jun-Wei Shao, Department of General Surgery, Wuhan General Hospital, Guangzhou Military Command of Chinese PLA, 801 Shuxiang Mendi, 76 Wuchang Lushi Road, Wuhan 430070, Hubei Province, China. sjwsjw555555@yahoo.com
Received: 2005-05-19 Accepted: 2005-06-08
Abstract
AIM: To construct the recombinant eukaryotic expression vector for human endostatin and express it in human liver cancer cell line SMMC-7721, and to observe its anti-angiogenesis activity in vitro.
METHODS: Human endostatin cDNA containing interleukin-2 (IL-2) secreting peptide was cloned into eukaryotic expression plasmid pcDNA3.0 to construct recombinant plasmid pCD-sEndo. The plasmid pCD-sEndo was transfected intoSMMC-7721 cells by cationic liposome. The positive cell clones were selected by G418 and named SMMC/sEndo ......
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