酵母双杂交技术筛选白细胞cDNA文库中新基因NS2TP蛋白结合蛋白基因
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张黎颖, 成 军, 邓 红, 郭 江, 郭风
病型肝炎病毒;酵母双杂交;NS2TP蛋白,张黎颖,成军,郭江,郭风劲,王巧侠,邓红,通讯作者,:,Screeningofpr
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张黎颖, 成军, 郭江, 郭风劲, 王巧侠 , 北京地坛医院传染病研究所 北京市 100011
邓红 , 西安交通大学第二医院感染科 陕西省西安市 710004
张黎颖, 女, 1978-10-26生, 陕西省西安市人, 汉族, 2002年毕业于西安交通大学临床医学系, 获医学学士学位, 2005年毕业于西安交通大学医学系, 获医学硕士学位, 主要研究方向是肝炎病毒与肝细胞相互作用的分子生物学机制.
国家自然科学基金攻关项目, No. C03011402, No. C30070689
军队“九、五”科技攻关项目, No. 98D063
军队回国留学人员启动基金项目, No. 98H038
军队“十、五”科技攻关青年基金项目, No. 01Q138
军队“十、五”科技攻关项目, No. 01MB135
通讯作者 : 成军, 100011, 北京安东城区安外大街地坛公园13号, 北京市地坛医院传染病研究所. cj@genetherapy.com.cn
电话: 010-64481639 传真: 010-64281540
收稿日期: 2005-01-10 接受日期: 2005-05-25
Screening of proteins binding to NS2TP from leukocyte cDNA library by yeast two-hybrid technique
Li-Ying Zhang, Jun Cheng, Hong Deng, Jiang Guo, Feng-Jin Guo, Qiao-Xia Wang
Li-Ying Zhang, Jun Cheng, Jiang Guo, Feng-Jin Guo, Qiao-Xia Wang, Institute of Infections Diseases, Ditan Hospital, Beijing 100011, China
Hong Deng, Darpartment of Infectious Diseases, the Second Hospital, Xi’an Jiaotong University Xi’an 710004, China
Supported by National Science Foundation of China, No.C03011402, No.C30070689; Returned Scholar Fundation of General Logistics Department of Chinese PLA, No. 98H038; the Science and Technique Foundation of Chinese PLA during the 9th and 10th Five-Year Period, No.98D063, No.01MB135; and the Science and Technique Foundation of Chinese PLA for Young Scholars during the 10th Five-Year Plan Period, No. 01Q138
Correspondence to: Dr. Jun Cheng, 13 Ditan Park, Anwai Street, Dongcheng District, Institute of Infectious Diseases, Ditan Hospital, Beijing 100011, China. cj@genetherapy.com.cn
Received: 2005-01-10 Accepted: 2005-05-25
Abstract
AIM: To screen the proteins binding to a transregulated protein of hepatitis C virus (HCV) NS2 protein (NS2TP) by yeast two-hybrid technique, and to investigate the pathogenesis of HCV and the biological functions of NS2TP ......
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