构象敏感凝胶电泳快速检测HBV准种序列异质性的方法
项目负责人,ArapidmethodofdetectionofhepatitisBvirusquesispeciesheterogeneitybyconformation-sensitivegelelectrophoresis,Correspondenceto:,Abstract,AIM:,METHODS:,RESULTS:,CONCLUSION:,摘要,目的:,方法:,结果:,结论:
兰林，王宇明，中国人民解放军第三军医大学西南医院全军感染病研究所 重庆市 400038兰林，男，1963-06-24生，四川省宜宾市人，1985年泸州医学院本科毕业，1994年华西医科大学硕士研究生毕业，2002年博士研究生，讲师. 主要从事传染性疾病的病毒学研究.
项目负责人 兰林，400038，重庆市，中国人民解放军第三军医大学西南医院全军感染病研究所. llin6624@hotmail.com
电话: 023-68757324 传真：023-68754475
收稿日期 2002-03-05 接受日期 2002-03-18
A rapid method of detection of hepatitis B virus quesispecies heterogeneity by conformation-sensitive gel electrophoresis
Lin Lan, Yu-Mimg Wang
Lin Lan, Yu-Mimg Wang, Institute of Infectious Diseases of PAL, Southwest Hospital,Third Military Medical University, Chongqing 400038, China
Correspondence to: Lin Lan, Institute of Infectious Diseases of PAL, Southwest Hospital, Third Military Medical University, Chongqing 400038, China. llin6624@hotmail.com
Received 2002-03-05 Accepted 2002-03-18
Abstract
AIM:To establish a method for detection of HBV quesispecies heterogeneity and to determine its sensibility and specificity.
METHODS:Base on the conformation-sensitive gel electrophoresis(CSGE)of HBV gene sequences, the authors changed the concentration of formamide and ethylene glycol and put urea in gel. Moreover, a preliminary CSGE was performed for selection of an optimal driver in each sample. Finally, 34 previously identified sequence plasmids that contained HBV C-ORF region were detected by CSGE with the improved conditions, and the results of CSGE were compared with that of nucleotide sequencing. ......
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