【摘要】 目的 建立阿昔洛韦血药浓度测定法，用于阿昔洛韦缓释片血药浓度研究。 方法 采用高效液相色谱法。分析柱为C 18 柱，流动相为2%乙腈水溶液(pH3)，检测波长254nm。 结果 样品组分在0.05～1.0μg/ml线性良好(0.9997)，最低血浆药物检测浓度为0.01μg/ml。回收率为92.36%～99.21%，日内及日间RSD<10%。 结论 本法简便、迅速、灵敏、准确，适合于临床药学研究需要。

    【关键词】 阿昔洛韦;血浆浓度;高效液相色谱法

    Determination of acyclovir in human plasma by HPLC

    GAO Dan，WU Gang.

    Harbin Pharm Group Sanchine Pharmaceutical Co，Ltd，Harbin150000，China

    【Abstract】 Objective To develop a precise，simple and convenient method for the determination of acyclovir con-centration in human plasma.Methods HPLC system was used.The analytical column was C 18 and the analytical mo-bile phase was acetonitrile water solution(2%).The detection wave-length was at254nm.Results The linear range of acyclovir in plasma was0.05～1.0μg/ml(0.9997).The detection limit was0.01μg/ml.The recovery was92.36%～99.21% ......