【摘要】 目的 探讨参麦注射液对急性缺血再灌注大鼠心肌细胞抗氧化能力的作用机制。 方法 结扎冠状动脉前降支复制大鼠心肌缺血再灌注损伤模型，测定心肌组织丙二醛(MDA)含量及结构型、诱导型一氧化氮合酶(cNOS，iNOS)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、血清肌酸激酶(CK)的活性。 结果 SM组心肌组织cNOS、SOD、GSH-Px活性高于MI/R组(P<0.01)，MDA含量、iNOS及血清CK活性低于MI/R组(P<0.01)。 结论 参麦注射液保护心肌缺血再灌注损伤的作用与其提高心肌抗氧化能力、清除氧自由基、减轻细胞膜脂质过氧化状态和维持细胞膜的完整性相关。

    【关键词】 参麦注射液;心肌缺血再灌注;抗脂质过氧化;大鼠

    Effect of on antioxidative ability of cardiomyocytes after acute ischemia-reperfu-sion in rats

    LI Ping，YANG Li，XIONG Fan，et al.

    Hubei College of Traditional Chinese Medicine，Wuhan430064，China

    【Abstract】 Objective To observe the effect of Shenma injection on antioxidative ability of cardiomyocytes after a-cute ischemia-reperfusion in rats.Methods The ischemia-reperfusion heart model was established by ligating/loosening the left anterior descending branch of coronary artery in Wistar rats.The activitie of cNOS，GSH-Px，SOD and the content of MDA，in the myocardium and CK activity of serum were examined.Results The activitie of cNOS，GSH-Px，SOD was increased significantly in the Shenma group as compared with that of ischemia-reperfu-sion group(P<0.01).CK ......