胎儿胰腺来源的单克隆SP细胞具有间充质的表型特征
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吴永华, 洪天配, 张 玲, 胡 江, 李凌松
单克隆SP细胞;胰腺干细胞;克隆;荧光激活细胞分选,吴永华,洪天配,张玲,胡江,李凌松,通讯作者,:,MonoclonalSPcellsderi
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吴永华,洪天配,张玲, 北京大学第三医院内分泌科 北京市 100083
胡江, 李凌松,北京大学干细胞研究中心 北京市 100083
吴永华,男,1977-01-26生,山西省太原市人,汉族,2002年北京大学硕士生.主要从事胰腺干细胞的应用基础研究.
国家自然科学基金项目,No. 30170443
211工程建设项目人体干细胞工程学学科群资助项目,No.108-2
通讯作者:洪天配,100083, 北京市花园北路49号,北京大学第三医院内分泌科. tpho66@bjmu.edu.cn
电话: 010-62017691-8212 传真:010-62017700
收稿日期:2005-05-08 接受日期:2005-06-13
Monoclonal SP cells derived from human fetal pancreas have phenotypicmarkers identical to mesenchymal stem cells
Yong-Hua Wu, Tian-Pei Hong, Ling Zhang, Jiang Hu, Ling-Song Li
Yong-Hua Wu, Tian-Pei Hong, Ling Zhang, Department ofEndocrinology, the Third Hospital of Peking University, Beijing 100083,China
Jiang Hu, Ling-Song Li, Research Center of Stem Cells, PekingUniversity, Beijing 100083, China
Supported by National Natural Science Foundation of China, No.30170443, and the 211 Project Foundation of Peking University, No.108-2
Correspondence to: Tian-Pei Hong, Department of Endocrinology, theThird Hospital of Peking University, Beijing 100083,China. tpho66@bjmu.edu.cn
Received: 2005-05-08 Accepted:2005-06-13
Abstract
AIM: To find out a new method for isolation and purification of humanpancreatic stem cells, and to investigate the characterization of thecells acquired by this method.
METHODS: Islet-like cell clusters (ICCs) were isolated from humanfetal pancreas. Monolayer epithelium-like cells were obtained from theICCs and passaged thereafter. The side population (SP) cells or non-SPcells were sorted from these cells at the sixth generation byfluorescence-activated cell sorting (FACS) ......
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