牛幽门螺杆菌抗体中和菌体蛋白对Hela细胞的生长抑制作用
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张世联, 刘 冉, 王 薇
牛抗HpyloriIgG;幽门螺杆菌;Hela细胞;SS1;NCTC11637,张世联,刘冉,王薇,通讯作者,:,Bovineanti-H
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张世联,刘冉,王薇, 河北省疾病预防控制中心医学研究部 河北省石家庄市050021
张世联,男,1956-10-01生,河北省沧州市人,1998年河北医科大学分子生物学专业硕士,研究员, 主要从事慢性疾病方面的应用基础研究.
河北省医学科研基金项目,No. 01118
通讯作者:张世联,050021, 河北省石家庄市青园街241号,河北省疾病预防控制中心医学研究部. zhangshl75@126.com
电话: 0311-86573208
收稿日期:2005-06-06 接受日期:2005-06-13
Bovine anti-Helicobacter pylori antibodies neutralize inhibitoryeffects of SS1 and NCTC11637 antigens on proliferation of Hela cells
Shi-Lian Zhang, Ran Liu, Wei Wang
Shi-Lian Zhang, Ran Liu, Wei Wang, Department of Medical Research,Hebei Center for Disease Prevention and Control, Shijiazhuang 050021,Hebei Province, China
Supported by the Medical Scientific Research Foundation of HebeiProvince, No.01118
Correspondence to: Dr. Shi-Lian Zhang, Department of MedicalResearch, Hebei Center for Disease Prevention and Control, 241 QingyuanStreet, Shijiazhuang 050021, Hebei Province,China. zhangshl75@126.com
Received: 2005-06-06 Accepted:2005-06-13
Abstract
AIM: To investigate the inhibitory effects of SS1 and NCTC11637antigens on the proliferation of Hela cells, and to observe theneutralization of bovine anti-Helicobacter pylori antibodiesagainst SS1 and NCTC11637.
METHODS: The cows were immunized with sonicated Helicobacterpylori (H pylori) antigen. After the scheduled immunization wascompleted, the immunization was strengthened with an interval of 1-2month.The bovine serum was prepared when the ratio in agar-doublediffusion was 1∶32.The bovine milk was collected at the same time. The serum proteins(81.4g/L) were isolated and purified by saturated ammonium sulfate (500,330, 330 g/L) and DEAE-32. The obtained proteins were identified by SDS-PAGE.Bovine anti-H pylori IgGs were incubated with SS1 or NCTC11637,respectively, at 37℃for 2 h, and then added into the solutions including Hela cells. Theproliferation of Hela cells was determined by cell growth curve. MTT assaywas adopted to evaluate the inhibitory effects of SS1 and NCTC11637antigens on the proliferation of Hela cells ......
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