http://www.100md.com 田 琴, 胡还忠, 马立群, 汪长东, 王晓敏,
田琴, 胡还忠,马立群, 汪长东, 王晓敏,梁华敏,华中科技大学同济医学院生理系 湖北省武汉市 430030
通讯作者:胡还忠,430030, 湖北省武汉市,华中科技大学同济医学院生理系. email@example.com
Effect of g-aminobutyricacid on spontaneous contraction of ileum smooth muscle in mice
Qin Tian, Huan-Zhong Hu, Li-Qun Ma, Chang-Dong Wang, Xiao-Min Wang, Hua-MinLiang
Qin Tian, Huan-Zhong Hu, Li-Qun Ma, Chang-Dong Wang, Xiao-Min Wang, Hua-MinLiang, Department of Physiology, Tongji Medical College, HuazhongUniversity of Science and Technology, Wuhan 430030, Hubei Province, China
Correspondence to: Huan-Zhong Hu, Department of Physiology, TongjiMedical College, Huazhong University of Science and Technology, Wuhan430030, Hubei Province, China. firstname.lastname@example.org
Received: 2005-06-06 Accepted: 2005-06-13
AIM: To investigate the effects of g-aminobutyricacid (GABA) on the spontaneous contraction of mouse ileac smooth muscleand the interactions between GABA and b-receptorand nitric oxide (NO).
METHODS: The contraction changes of the isolated smooth musclestripswere recorded by tension transducer, and the change of the tensionwas used as the marker to evaluate the effects of GABA. The effects of ODQ,L-NNA, propranolol on the action of GABA were also analyzed. .
RESULTS: GABA inhibited the spontaneous contraction of mouse ileumat the concentrations ranging from 1×10-6to 1×10-3mol/L. The contraction amplitudes were decreased by (34.71±7.35)%and (22.23±4.69)%,respectively, when 1×10-6and 1×10-3mol/L GABA were used. Picrotoxin showed no significant effect on theinhibitory actions caused by GABA. The effects of GABA on the spontaneouscontraction were decreased in the presence of L-NNA or ODQ. L-Arg (5×10-7mol/L) decreased the effect of GABA at the concentration of 1×10-6mol/L, but not at the concentration of 1×10-3mol/L. Propranolol (3×10-6mol/L) reduced the effect of GABA on the contraction of mouse ileac smoothmuscle.
CONCLUSION: GABA inhibits the spontaneous contraction of mouseileac smooth muscle. This effect, which is influenced by the excitation ofb-receptormay need the participation of cGMP and NO.
Key Words: g-aminobutyricacid; Nitric oxide; b receptor; Smooth muscle; Ileum
Tian Q, Hu HZ, Ma LQ, Wang CD, Wang XM, Liang HM. Effect of g-aminobutyricacid on spontaneous contraction of ileum smooth muscle in mice. ShijieHuaren Xiaohua Zazhi 2005;13(16):1981-1984
结果:GABA抑制小鼠回肠平滑肌自主收缩活动.1×10-6和1×10-3mol/L GABA抑制率分别为34 ......