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GABA对小鼠回肠平滑肌自主收缩活动的影响
http://www.100md.com 田 琴, 胡还忠, 马立群, 汪长东, 王晓敏,
GABA;NO;b受体;平滑肌;回肠,田琴,胡还忠,马立群,汪长东,王晓敏,梁华敏,田琴,通讯作者,:,Effectof,g,-aminobutyric
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     田琴, 胡还忠,马立群, 汪长东, 王晓敏,梁华敏,华中科技大学同济医学院生理系 湖北省武汉市 430030

    田琴, 女,1980-10-19生,湖北省恩施人,土家族,华中科技大学同济医学院生理学硕士,主要从事消化生理方面的研究.

    通讯作者:胡还忠,430030, 湖北省武汉市,华中科技大学同济医学院生理系. huhuanzh@163.com

    电话: 027-83630850

    收稿日期:2005-06-06 接受日期:2005-06-13

    Effect of g-aminobutyricacid on spontaneous contraction of ileum smooth muscle in mice

    Qin Tian, Huan-Zhong Hu, Li-Qun Ma, Chang-Dong Wang, Xiao-Min Wang, Hua-MinLiang

    Qin Tian, Huan-Zhong Hu, Li-Qun Ma, Chang-Dong Wang, Xiao-Min Wang, Hua-MinLiang, Department of Physiology, Tongji Medical College, HuazhongUniversity of Science and Technology, Wuhan 430030, Hubei Province, China

    Correspondence to: Huan-Zhong Hu, Department of Physiology, TongjiMedical College, Huazhong University of Science and Technology, Wuhan430030, Hubei Province, China. huhuanzh@163.com

    Received: 2005-06-06 Accepted: 2005-06-13

    Abstract

    AIM: To investigate the effects of g-aminobutyricacid (GABA) on the spontaneous contraction of mouse ileac smooth muscleand the interactions between GABA and b-receptorand nitric oxide (NO).

    METHODS: The contraction changes of the isolated smooth musclestripswere recorded by tension transducer, and the change of the tensionwas used as the marker to evaluate the effects of GABA. The effects of ODQ,L-NNA, propranolol on the action of GABA were also analyzed. .

    RESULTS: GABA inhibited the spontaneous contraction of mouse ileumat the concentrations ranging from 1×10-6to 1×10-3mol/L. The contraction amplitudes were decreased by (34.71±7.35)%and (22.23±4.69)%,respectively, when 1×10-6and 1×10-3mol/L GABA were used. Picrotoxin showed no significant effect on theinhibitory actions caused by GABA. The effects of GABA on the spontaneouscontraction were decreased in the presence of L-NNA or ODQ. L-Arg (5×10-7mol/L) decreased the effect of GABA at the concentration of 1×10-6mol/L, but not at the concentration of 1×10-3mol/L. Propranolol (3×10-6mol/L) reduced the effect of GABA on the contraction of mouse ileac smoothmuscle.

    CONCLUSION: GABA inhibits the spontaneous contraction of mouseileac smooth muscle. This effect, which is influenced by the excitation ofb-receptormay need the participation of cGMP and NO.

    Key Words: g-aminobutyricacid; Nitric oxide; b receptor; Smooth muscle; Ileum

    Tian Q, Hu HZ, Ma LQ, Wang CD, Wang XM, Liang HM. Effect of g-aminobutyricacid on spontaneous contraction of ileum smooth muscle in mice. ShijieHuaren Xiaohua Zazhi 2005;13(16):1981-1984

    摘要

    目的:观察GABA对小鼠回肠平滑肌运动功能的影响,探讨GABA的作用与b受体、NO之间的关系.

    方法:以离体回肠收缩张力的变化为指标,观察GABA的作用,GABAA受体抑制剂木防己苦毒素picrotoxin,b受体的阻断剂propranolol,NOS抑制剂L-NNA,cGMP合成酶的抑制剂ODQ对GABA作用的影响.

    结果:GABA抑制小鼠回肠平滑肌自主收缩活动.1×10-6和1×10-3mol/L GABA抑制率分别为34 ......

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