重症胰腺炎肾脏损伤中细胞凋亡相关基因的作用
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朱明德, 方驰华, 史学深
重症胰腺炎;Bax;Bcl-2;肾细胞凋亡,朱明德，方驰华，史学深，,朱明德,通讯作者：,RenalcellsapoptosisandBax,Bcl-2e
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朱明德，方驰华，史学深，南方医科大学珠江医院肝胆外科 广东省广州市 510282
朱明德, 男，1970-11-25生，湖北省武汉市人，汉族，2003年南方医科大学硕士生，主要从事肝胆胰外科疾病的研究.
通讯作者：方驰华, 510282，广东省广州市工业大道中，珠江医院普外科. fch58520@sina.com
收稿日期: 2005-06-28 接受日期: 2005-07-08
Renal cells apoptosis and Bax, Bcl-2 expression in rats with severe acute pancreatitis
Ming-De Zhu, Chi-Hua Fang, Xue-Shen Shi
Ming-De Zhu, Chi-Hua Fang, Xue-Shen Shi, Department of Hepatobiliary Surgery, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China
Correspondence to: Chi-Hua Fang, Department of General Surgery, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China. fch58520@sina.com
Received: 2005-06-28 Accepted: 2005-07-08
Abstract
AIM: To explore the roles of renal cell apoptosis and expression of Bax and Bcl-2 in renal injury in rats with severe acute pancreatitis (SAP).
METHODS: Thirty rats were randomized into sham operation (SO, n = 15) and SAP group (n = 15). The model of SAP was established by retrograde injection of 50 g/L sodium taurocholate into the biliopancreatic duct in the rats. Serum creatinine (Cr) and blood urea nitrogen (BUN) were determined 6, 12, and 18 h after injection. The pathological changes were observed by light and electron microscopy. The apoptosis of renal cells was determined by TUNEL method, and the expression of Bax and Bcl-2 was detected by immunohistochemical staining (SABC assay).
RESULTS: As compared with those in SO group at the same phase, serum Cr and BUN in SAP group increased markedly. Significant injuries of renal tissue were observed under light and electron microscope.The apoptotic index of renal glomerular cells increased significantly 6, 12, 18 h after injection (3.2±1.1 vs 0.8±0.1, P <0.05; 3.9±1.8vs 0.9±0.1, P <0.05; 4.8±1.7 vs 0.8±0.2, P <0.01, respectively). The apoptotic index of renal tubule cells also increased significantly (9 ......
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