刘东屏，于继红，王炳元，中国医科大学附属第一医院消化内科 辽宁省沈阳市 110001
李茵茵，鞍山曙光医院肿瘤内科 辽宁省鞍山市 114000
刘东屏, 女, 1963-11-14生, 辽宁省辽阳市人, 汉族, 1985年中国医科大学本科毕业, 1988年硕士研究生, 1998年博士研究生, 副教授, 硕士研究生导师, 主要从事消化道肿瘤的研究.
通讯作者：刘东屏, 110001, 沈阳市和平区南京北街155号, 中国医科大学附属第一医院消化内科. firstname.lastname@example.org
Relationship between expression of Epstein-Barr virus latent membraneprotein and H pylori infection in gastric mucosa withintestinal metaplasia
Dong-Ping Liu, Ji-Hong Yu, Yin-Yin Li, Bing-Yuan Wang
Dong-Ping Liu, Ji-Hong Yu, Bing-Yuan Wang, Department ofGastroenterology, the First Affiliated Hospital of China MedicalUniversity, Shenyang 110001, Liaoning Province, China
Yin-Yin Li, Department of Oncology, Shuguang Hospital of Anshan,Anshan 114000, Liaoning Province, China
Correspondence to: Dong-Ping Liu, Department of Gastroenterology,the First Affiliated Hospital of China Medical University, Shenyang110001, Liaoning Province, China. email@example.com
AIM: To observe the expression of Epstein-Barr virus latentmembrane protein(EBV-LMP) in different types of intestinalmetaplasia(IM), and to explore its relation with H pylori infection.
METHODS: One hundred and seventy-one patients with IM were diagnosedusing HE staining, and 30 normal cases were selected as controls.HID-AbpH2.5-PAS mucoprotein histochemical staining and SP histochemicalstaining were used to determine the subtypes of the IM and EBV-LMP,respectively. H pylori infection was examined by HE staining, Hpylori-DNA polymerase chain reaction, and enzyme linkedimmunosorbent assay.
RESULTS: There was no expression of EBV-LMP in the normal gastricmucosa, while the positive rate of EBV-LMP in the IM ones was 33.3% andthe EBV-LMP was mainly stained in the cell nuclei. The expression ofEBV-LMP in the IM mucosa was significantly higher than that in normalones(P = 0.000 187). The rate of H pylori infection was63.7% in the IM mucosa, which was correlated with IM (rs =0.275, P = 0.000 755). The rate of H pylori infection inthe IM mucosa with EBV-LMP expression was 63.2% (36/57), which wassignificantly higher than that in normal mucosa(P = 0.000 445). Hpylori infection was significantly related to the expression ofEBV-LMP in the IM mucosa (rs = 0.522, P = 0.000001)..
CONCLUSION: EBV-LMP is expressed in IM mucosa, and latent infectionof EBV exists in some of them. The expression of EBV-LMP issignificantly higher in each subtype of the IM mucosa than that in thenormal ones. H pylori expression is correlated with theexpression of EBV-LMP in IM mucosa.
Key Words: Epstein-Barr virus latent membrane protein;Intestinal metaplasia; H pylori
Liu DP, Yu JH, Li YY, Wang BY. Relationship between expression ofEpstein-Barr virus latent membrane protein and H pylori infectionin gastric mucosa with intestinal metaplasia ......