甜菜碱对酒精性肝损伤大鼠凋亡基因caspase-12表达的影响
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张 频, 龚作炯
酒精性肝损伤; 甜菜碱; caspase-12,张频, 龚作炯,张频,通讯作者:,Effects
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张频, 龚作炯, 武汉大学人民医院感染科 湖北省武汉市 430060
张频, 女, 1979-09-23生, 湖北省荆州市人, 汉族, 2004年武汉大学医学院硕士研究生, 主要从事肝脏疾病的研究.
通讯作者: 龚作炯, 湖北省武汉市, 430060, 武汉大学人民医院感染科. zjgong@163.com
电话: 027-88041911-8385
收稿日期: 2005-09-05 接受日期: 2005-09-30
Effects of betaine on expression of caspase-12 in ethanol-induced liver injury in rats
Pin Zhang, Zuo-Jiong Gong
Pin Zhang, Zuo-Jiong Gong, Department of Infectious Diseases, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China
Correspondence to: Dr. Zuo-Jiong Gong, Department of Infectious Diseases, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China. zjgong@163.com
Received: 2005-09-05 Accepted: 2005-09-30
Abstract
AIM: To investigate the effects of betaine on the expression of caspase-12 in the liver of rats with ethanol-induced liver injury.
METHODS: Forty-eight female SD rats were randomly divided into 4 groups: control, model, and low and high dose betaine group. Except the rats in control group, all the rats were fed fat-rich diet plus ethanol plus fish oil gavage for 8 wk. Betaine was administered intragastrically in the rats of betaine groups after 4-week exposure of ethanol. The expression of caspase-12 was detected in the liver tissues by immunohistomistry and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
RESULTS: Compared with that in control group, the expression of caspase-12 was markedly induced in the liver after chronic ethanol consumption (mRNA: 1.00 vs 0.18, P <0.01; protein: 0.296 9±0.045 1 vs 0.052 6±0.023 4, P <0.01). However, the level of caspa-se-12 expression was significantly lower in high- and low-dose betaine group than that in model group (mRNA: 0.10, 0.12 vs 1.00, P <0.01; protein: 0.121 5±0.013 0, 0 ......
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