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甜菜碱对酒精性肝损伤大鼠凋亡基因caspase-12表达的影响
http://www.100md.com 张 频, 龚作炯
酒精性肝损伤; 甜菜碱; caspase-12,张频, 龚作炯,张频,通讯作者:,Effects
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     张频, 龚作炯, 武汉大学人民医院感染科 湖北省武汉市 430060

    张频, 女, 1979-09-23生, 湖北省荆州市人, 汉族, 2004年武汉大学医学院硕士研究生, 主要从事肝脏疾病的研究.

    通讯作者: 龚作炯, 湖北省武汉市, 430060, 武汉大学人民医院感染科. zjgong@163.com

    电话: 027-88041911-8385

    收稿日期: 2005-09-05 接受日期: 2005-09-30

    Effects of betaine on expression of caspase-12 in ethanol-induced liver injury in rats

    Pin Zhang, Zuo-Jiong Gong

    Pin Zhang, Zuo-Jiong Gong, Department of Infectious Diseases, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China

    Correspondence to: Dr. Zuo-Jiong Gong, Department of Infectious Diseases, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China. zjgong@163.com

    Received: 2005-09-05 Accepted: 2005-09-30

    Abstract

    AIM: To investigate the effects of betaine on the expression of caspase-12 in the liver of rats with ethanol-induced liver injury.

    METHODS: Forty-eight female SD rats were randomly divided into 4 groups: control, model, and low and high dose betaine group. Except the rats in control group, all the rats were fed fat-rich diet plus ethanol plus fish oil gavage for 8 wk. Betaine was administered intragastrically in the rats of betaine groups after 4-week exposure of ethanol. The expression of caspase-12 was detected in the liver tissues by immunohistomistry and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR).

    RESULTS: Compared with that in control group, the expression of caspase-12 was markedly induced in the liver after chronic ethanol consumption (mRNA: 1.00 vs 0.18, P <0.01; protein: 0.296 9±0.045 1 vs 0.052 6±0.023 4, P <0.01). However, the level of caspa-se-12 expression was significantly lower in high- and low-dose betaine group than that in model group (mRNA: 0.10, 0.12 vs 1.00, P <0.01; protein: 0.121 5±0.013 0, 0.185 0±0.008 5 vs 0.296 9±0.045 1, P <0.01). Significant difference of caspase-12 expression also existed between high- and low-dose betaine group (mRNA: 0.10 vs 0.12, P <0.05; protein: 0.121 5±0.013 0 vs 0.185 0±0.008 5, P <0.05).

    CONCLUSION: Betaine suppresses the expression of caspase-12 both in mRNA and protein level in the rats with ethanol-induced liver injury, which may be related to its anti-apoptotic mechanism.

    Key Words: Ethanol-induced liver; Betaine; caspase-12

    Zhang P, Gong ZJ. Effects of betaine on expression of caspase-12 in ethanol-induced liver injury in rats. Shijie Huaren Xiaohua Zazhi 2005;13(20):2437-2440

    摘要

    目的:研究甜菜碱对凋亡基因caspase-12表达的影响, 探讨其抗酒精性肝损伤(ALD)大鼠肝细胞凋亡的机制.

    方法:
采用酒精加鱼油灌胃配合高脂饮食建立酒精性肝损伤模型, 应用半定量逆转录聚合酶链反应(RT-PCR)法和免疫组化法检测大鼠肝组织凋亡基因caspase-12 mRNA和蛋白水平.

    结果:
与正常组比较, 模型组大鼠肝组织caspase-12表达明显增强(mRNA: 1.00 vs 0.18, P<0 ......

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