清热化湿法对温病湿热证大鼠肝脏LDL-R mRNA的影响
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吕文亮, 高清华, 李华锋
清热化湿法;温病湿热证;LDL-RmRNA;大鼠,清热化湿法对温病湿热证大鼠肝脏LDL-RmRNA的影响,吕文亮,李华锋,高清华,通讯作者:,EffectofQingreHuashirecipeo
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吕文亮, 李华锋, 湖北中医学院中医系 湖北省武汉市 430061
高清华, 湖北中医学院病原病理实验室 湖北省武汉市 430061
湖北省教育厅青年基金重点资助项目, No.2003A003
通讯作者: 吕文亮, 430061, 湖北省武汉市武昌区, 湖北中医学院中医系. lvwenliang66@163.com
电话: 027-68889091
收稿日期: 2005-06-21 接受日期: 2005-07-08
Effect of Qingre Huashi recipe on liver low density lipoprotein-receptor mRNA in rats with epidemic febrile disease of dampness-heat syndrome
Wen-Liang Lv, Qing-Hua Gao, Hua-Feng Li
Wen-Liang Lv, Hua-Feng Li, Department of Traditional Chinese Medicine, Hubei College of Traditional Chinese Medicine, Wuhan 430061, Hubei Province, China
Qing-Hua Gao, Laboratory of Pathogen and Pathology, Hubei College of Traditional Chinese Medicine, Wuhan 430061, Hubei Province, China
Supported by the Key Foundation for Youths of Education Office of Hubei Province, No.2003A003
Correspondence to: Wen-Liang Lv, Department of Traditional Chinese Medicine, Hubei College of Traditional Chinese Medicine, Wuchang District, Wuhan 430061, Hubei Province, China.
lvwenliang66@163.com
Received: 2005-06-21 Accepted: 2005-07-08
Abstract
AIM: To establish the rat models of dampness-heat syndrome by stimulation of compound factors, and to investigate the effect of Qingre Huashi recipe on the expression of low density lipoprotein-receptor (LDL-R) mRNA in the liver tissues of rats.
METHODS: Forty-eight male Wistar rats were randomly divided into six groups: normal control, dampness-heat syndrome model, Qingre Huashi (A), Qingre Jiedu (B), Xuanqi Huashi (C), and Lipanthyl (D) group. Each group contained 8 rats. Except the rats in the normal control group, the ones in other groups were all treated with the fat and sweet diet plus artificial climate box plus Salmonella typhimurtum to establish the model of dampness-heat syndrome. Then the rats in A, B, C, D group were treated the corresponding medicine for 5 d. The expression of LDL-R mRNA was detected in the liver tissues of rats by Using reverse transcription polymerase chain reaction ......
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