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磷酸化黏着斑激酶在结肠癌中表达及意义
http://www.100md.com 刘启胜, 于红刚, 齐元玲, 曹 俊, 罗和生,
结肠癌;黏着斑激酶;磷酸化,刘启胜,于红刚,齐元玲,曹俊,罗和生,于皆平,通讯作者:,Expressionandsignificanceofphosphoryl
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     刘启胜, 于红刚, 齐元玲, 曹俊, 罗和生, 于皆平, 武汉大学人民医院消化内科 湖北省武汉市 430060

    国家自然科学基金资助项目, No.30470782

    通讯作者: 于红刚, 430060, 湖北省武汉市, 武汉大学人民医院消化内科. wuzhixuan2003@126.com

    电话: 027-88076874

    收稿日期: 2005-09-28 接受日期: 2005-09-30

    Expression and significance of phosphorylated focal adhesion kinase in colon carcinoma

    Qi-Sheng Liu, Hong-Gang Yu, Yuan-Ling Qi, Jun Cao, He-Sheng Luo, Jie-Ping Yu

    Qi-Sheng Liu, Hong-Gang Yu, Yuan-Ling Qi, Jun Cao, He-Sheng Luo, Jie-Ping Yu, Department of Gastroenterology, People’s Hospital of Wuhan University, Wuhan 430060, Hubei Province, China

    Supported by National Natural Science Foundation of China, No. 30470782

    Correspondence to: Hong-Gang Yu, Department of Gastroenterology, People’s Hospital of Wuhan University, Wuhan 430060, Hubei Province, China. wuzhixuan2003@126.com

    Received: 2005-09-28 Accepted: 2005-09-30

    Abstract

    AIM: To investigate the expression of phosphorylated focal adhesion kinase (phospho-FAK) and its signific-ance in human colon carcinoma.

    METHODS: The phospho-FAK (including FAK) expre-ssion was detected by Western bloting in 20 cases of colon carcinoma and their corresponding para-cancer tissues.

    RESULTS: The positive rate of FAK expression in the cancer tissues was significantly higher than that in the corresponding normal tissues (95% vs 60%, c2 = 5.16, P <0.05). The mean level of FAK expression in the cancer tissues was 0.482±0.150, while the mean level of expression in the normal tissue was 0.269±0.015 (t = 6.39, P <0.01). The positive rate of Tyr-397 FAK protein expression in the cancer tissues was 90%, while the positive rate in the corresponding normal tissues was only 20% (c2 = 17.1, P <0.01). The mean level of Tyr-397 FAK protein expression in the cancer tissue was notably higher than that in the corresponding para-cancer tissues (0.385±0.021 vs 0.110±0.005, t = 54.23, P <0.01).

    CONCLUSION: The up-regulation of FAK expression, especially Tyr-397 FAK protein expression, may play an important role in the tumorigenesis and progression of colon carcinoma.

    Key Words: Colon carcinoma; FAK; Phosphorylation

    Liu QS, Yu HG, Qi YL, Cao J, Luo HS, Yu JP. Expression and significance of phosphorylated focal adhesion kinase in colon carcinoma. Shijie Huaren Xiaohua Zazhi 2005;13(20):2490-2493

    摘要


    目的:观察磷酸化黏着斑激酶(phosphorylated focal adhesion kinase, phospho-FAK)在结肠癌组织和对应癌旁组织中的表达, 探讨其在结肠癌发病的可能机制.

    

    方法:
采用Western bloting方法检测20例新鲜结肠癌及相对应的癌旁组织FAK表达水平, 并在调平每对组织FAK的含量后再进行FAK Tyr397磷酸化蛋白的检测.

    结果:
20例结肠癌组织FAK阳性表达率为95%, 对应癌旁组织FAK阳性表达率为60%(χ2 = 5.16, P<0.05); 癌组织表达平均值为0.482±0.150, 癌旁表达平均值为0.269±0.015(t = 6.39,P<0.01). 20例结肠癌组织18例有FAK Tyr397磷酸化蛋白表达, 表达率为90%, 而对应癌旁组织仅有4例有FAK Tyr397磷酸化蛋白表达, 表达率为20%(χ2 = 17.1, P<0.01); 癌组织表达平均值为0.385±0.021, 癌旁表达平均值为0.110±0.005(t = 54 ......

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